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1、ActaBiomaterialia7(2011)959–966ContentslistsavailableatScienceDirectActaBiomaterialiajournalhomepage:www.elsevier.com/locate/actabiomatPEG-basedhydrogelswithtunabledegradationcharacteristicstocontroldeliveryofmarrowstromalcellsfortendonoveruseinjuriesaaaaaa,b,⇑YongzhiQiu,JeremyJ.Lim,
2、LarryScottJr.,RyanC.Adams,HieuT.Bui,JohnnaS.TemenoffaW.H.CoulterDepartmentofBiomedicalEngineering,GeorgiaInstituteofTechnologyandEmoryUniversity,313FerstDrive,Atlanta,GA30332,USAbPetitInstituteforBioengineeringandBioscience,GeorgiaInstituteofTechnology,315FerstDrive,Atlanta,GA30332,U
3、SAarticleinfoabstractArticlehistory:Marrowstromalcells(MSCs)havebeensuggestedasameanstoimprovehealingintendonoveruseinju-Received21July2010ries(tendinopathy),butoptimaldeliverymethodsforthesecellshaveyettobedetermined.InthisstudyReceivedinrevisedform28Septembernoveldegradablehydrogel
4、sbasedonoligo(poly(ethyleneglycol)fumarate)(OPF)andacrylatedpoly(eth-2010yleneglycol)–dithiothreitol(AcPEG–DTT)withtunabledegradationtimesrangingfromafewdaystoAccepted1November2010>1monthweresynthesizedasMSCcarriersfortendonoveruseinjuries.TheadditionofhigheramountsAvailableonline4No
5、vember2010ofOPForhigherdithiothreitol(DTT)concentrationsresultedinenhancedfoldswellinganddegradation.Threeformulations,includingnon-degrading,slowerdegrading(degradedin10days)andfasterKeywords:degrading(degradedin5days)hydrogelswereselectedforstudieswithMSCsintendontissueexplantsHy
6、drogelMarrowstromalcellsthathadbeentreatedwithcollagenaseasareproduciblemodeloftendinopathy.QuantitativeanalysisofCellinfiltrationtheresultinghistologyimagesindicatedthatcelldeliveryfromthehydrogelswasdependentonthedeg-Tendon/ligamentradationrate,withcellspresentinthetissueonlyafterhy
7、drogeldissolution.Inaddition,significantlyTendinopathymorecellswerefoundinthetendonafter14dayswiththefastdegrading(53±19)vs.slowdegrading(20±6)hydrogels.Basedontheseresults,OPF/AcPEG–DTThydrogelsprovideaversatilebiomaterialplatformtocontrolcelldeliveryandthusbetteridentifydosingregime
8、nsrequiredfo
