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《Global Phosphoproteomic Analysis Reveals Diverse Functions of Serine Threonine Tyrosine Phosphorylation in the Model Cyanobacterium Synechococcus sp. Strain PCC 7002》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、Articlepubs.acs.org/jprGlobalPhosphoproteomicAnalysisRevealsDiverseFunctionsofSerine/Threonine/TyrosinePhosphorylationintheModelCyanobacteriumSynechococcussp.StrainPCC7002††,‡†††,†,†Ming-kunYang,Zhi-xianQiao,Wan-yiZhang,QianXiong,JiaZhang,TaoLi,*FengGe,*,†andJin-dong
2、Zhao*†KeyLaboratoryofAlgalBiology,InstituteofHydrobiology,ChineseAcademyofSciences,Wuhan,430072,China‡UniversityofChineseAcademyofSciences,Beijing100039,China*SSupportingInformationABSTRACT:Increasingevidenceshowsthatproteinphos-phorylationonserine(Ser),threonine(Thr
3、),andtyrosine(Tyr)residuesisoneofthemajorpost-translationalmodificationsinthebacteria,involvedinregulatingamyriadofphysiologicalprocesses.Cyanobacteriaareoneofthelargestgroupsofbacteriaandaretheonlyprokaryotescapableofoxygenicphotosynthesis.Manycyanobacteriastrainscon
4、tainunusuallyhighnumbersofproteinkinasesandphosphataseswithspecificityonSer,Thr,andTyrresidues.However,onlyafewdozenphosphorylationsitesincyanobacteriaareknown,presentingamajorobstacleforfurtherunderstandingtheregulatoryrolesofreversiblephosphorylationinthisgroupofbac
5、teria.Inthisstudy,wecarriedoutaglobalandsite-specificphosphoproteomicanalysisonthemodelcyanobacteriumSynechococcussp.PCC7002.Intotal,280phosphopeptidesand410phosphorylationsitesfrom245Synechococcussp.PCC7002proteinswereidentifiedthroughthecombineduseofprotein/peptidepr
6、efractionation,TiO2enrichment,andLC−MS/MSanalysis.Theidentifiedphosphoproteinswerefunctionallycategorizedintoaninteractionmapandfoundtobeinvolvedinvariousbiologicalprocessessuchastwo-componentsignalingpathwayandphotosynthesis.Ourdataprovidethefirstglobalsurveyofphospho
7、rylationincyanobacteriabyusingaphosphoproteomicapproachandsuggestawide-rangingregulatoryscopeofthismodification.TheprovideddatasetmayhelprevealthephysiologicalfunctionsunderlyingSer/Thr/Tyrphosphorylationandfacilitatetheelucidationoftheentiresignalingnetworksincyanoba
8、cteria.KEYWORDS:Cyanobacteria,Synechococcussp.PCC7002,phosphoproteomics,two-componentsignalingpathway,photosynthesis■INTRODUCTIONRe
