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《转录的初始化》ppt课件

《转录的初始化》ppt课件

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时间：2019-07-21

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1、Chapter20Initiationoftranscription20.1Introduction 20.2EukaryoticRNApolymerasesconsistofmanysubunits 20.3Promoterelementsaredefinedbymutationsandfootprinting 20.4RNApolymeraseIhasabipartitepromoter 20.5RNApolymeraseIIIusesbothdownstreamandupstreampromoters 20.6ThestartpointforRNApolymeraseII

2、 20.7TBPisauniversalfactor 20.8TBPbindsDNAinanunusualway 20.9Thebasalapparatusassemblesatthepromoter 20.10Initiationisfollowedbypromoterclearance 20.11Aconnectionbetweentranscriptionandrepair 20.12PromotersforRNApolymeraseIIhaveshortsequenceelements 20.13Somepromoter-bindingproteinsarerepres

3、sors 20.14Enhancerscontainbidirectionalelementsthatassistinitiation 20.15IndependentdomainsbindDNAandactivatetranscription 20.16Thetwohybridassaydetectsprotein-proteininteractions 20.17InteractionofupstreamfactorswiththebasalapparatusEnhancerelementisacis-actingsequencethatincreasestheutiliz

4、ationof(some)eukaryoticpromoters,andcanfunctionineitherorientationandinanylocation(upstreamordownstream)relativetothepromoter.20.1IntroductionFigure20.1AtypicalgenetranscribedbyRNApolymeraseIIhasapromoterthatextendsupstreamfromthesitewheretranscriptionisinitiated.Thepromotercontainsseveralsh

5、ort(<10bp)sequenceelementsthatbindtranscriptionfactors,dispersedover>200bp.Anenhancercontainingamorecloselypackedarrayofelementsthatalsobindtranscriptionfactorsmaybelocatedseveralkbdistant.(DNAmaybecoiledorotherwiserearrangedsothattranscriptionfactorsatthepromoterandattheenhancerinteracttofo

6、rmalargeproteincomplex.)20.1IntroductionAmanitin(morefullya-amanitin)isabicyclicoctapeptidederivedfromthepoisonousmushroomAmanitaphalloides;itinhibitstranscriptionbycertaineukaryoticRNApolymerases,especiallyRNApolymeraseII.20.2EukaryoticRNApolymerasesconsistofmanysubunitsFigure20.2Eukaryotic

7、RNApolymeraseIIhas>10subunits.20.2EukaryoticRNApolymerasesconsistofmanysubunitsCotransfectionisthesimultaneoustransfectionoftwomarkers.20.3PromoterelementsaredefinedbymutationsandfootprintingFigure20.3Promoterboundariescanbedeterminedbymakingdeleti

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