资源描述：
《Reversible MCAO without craniectomy in rats.》由会员上传分享，免费在线阅读，更多相关内容在学术论文-天天文库。
1、ReversiblemiddlecerebralarteryocclusionwithoutcraniectomyinratsEZLonga,PRWeinstein,SCarlsonandRCumminsStroke1989;20;84-91StrokeispublishedbytheAmericanHeartAssociation.7272GreenvilleAvenue,Dallas,TX7251484ReversibleMiddleCerebralArteryOcclusionWithoutCraniectomyinRatsEnriqueZeaLonga,MD,PhilipR.We
2、instein,MD,SaraCarlson,BS,andRobertCummins,MSTodevelopasimple,relativelynoninvasivesmall-animalmodelofreversibleregionalcerebralischemia,wetestedvariousmethodsofinducinginfarctionintheterritoryoftherightmiddlecerebralartery(MCA)byextracranjalvascularocclusioninrats.Inpreliminarystudies,60ratswere
3、anesthetizedwithketamineanddifferentcombinationsofvesselswereoccluded;bloodpressureandarterialbloodgasesweremonitored.Neurologicdeficit,mortalityrate,grosspathology,andinsomeinstances,electroencephalogramandhistochemicalstainingresultswereevaluatedinallsurvivingrats.Theprincipalprocedureconsisted
4、ofintroducinga4-0nylonintraluminalsutureintothecervicalinternalcarotidirtery(ICA)andadvancingitintracra-niallytoblockbloodflowintotheMCA;collateralbloodflowwasreducedbyinterruptingallbranchesoftheexternalcarotidartery(ECA)andallextracranialbranchesoftheICA.Insomegroupsofrats,bilateralvertebralorc
5、ontralateralcarotidarteryocclusionwasalsoperformed.Indiainkperfusionstudiesin20ratsdocumentedblockageofMCAbloodflowin14ratssubjectedtopermanentocclusionandtherestorationofbloodflowtotheMCAterritoryinsixratsafterwithdrawalofthesuturefromtheICA.ThebestmethodofMCAocclusionwasthenselectedforfurtherco
6、nfirmatorystudies,includinghistologicexamination,infiveadditionalgroupsofratsanesthetizedwithhalothane.SevenofeightratsthatunderwentpermanentocclusionoftheMCAhadresolvingmoderatelysevereneurologicdeficits(Grade2of4)andunilateralinfarctsaveraging37.6±5.5%ofthecoronalsectionalareaat72hoursaftertheo
7、nsetofocclusion.Fiveratsunderwentthesameprocedureafterbilateralvertebralarteryocclusionwasperformedtoreducecollateralbloodflow.Onlytwoofthesefiveratssurvived72hours;theneurologicdeficitsprogressedfromGrade2.5to3,andthe
