基因芯片的生物信息学

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1、MicroarrayandBioinformatics基因芯片的生物信息学DrJingfuQiu邱景富SchoolofPublicHealth公共卫生学院AimsfortheMicroarrayBioinformaticsUnderstandbasicmicroarraytechnologyanditsuseingeneexpressionanalysis.基因芯片技术与表达谱分析中的应用Learnbasicdataanalysismethodsandhowtoapplythemintheanalysisofgeneexpression

2、data基因芯片的数据分析Dataacquisition数据获得Datanormalization数据归一化Dataanalysis数据分析DataClustering数据聚类Vocabulary-Review回顾Gene基因:hereditaryDNAsequenceataspecificlocationonchromosome.Genetics遗传学:studyofheredity&variationinorganisms.Genome基因组:anorgan'stotalcontent(fullDNAsequence)Genomic

3、s基因组学:studyoforganismsintermsoftheirgenome.2002年2月12日,历时10载耗资20亿美元的人类基因组计划最终完成,并报道了99%的人类基因组序列.Vocabulary-Review回顾Protein蛋白质:sequenceofaminoacidsthat“doessomething”Proteomics蛋白质组学:studyofalloftheproteinsthatcancomefromanorganismsgenomeBioinformatics生物信息学:thecollection,or

4、ganization&analysisoflarge-scale,complexbiologicaldata.FunctionalGenomics功能基因组学:studyofobtaininganoverallpictureofgenomefunctions,includingtheexpressionprofilesatthemRNAlevelandtheproteinlevelMicroarray基因芯片–AhighthroughputtechnologythatallowsdetectionofthousandsofgenesSi

5、multaneously–genechip,biochip,array–Muchrelyoncomputeraids–CentralplatformforfunctionalgenomicsTypesofMicroarrays芯片的种类DNAmicroarrays,suchascDNAmicroarraysandoligonucleotidemicroarraysMMChips,forsurveillanceofmicroRNApopulationsProteinmicroarraysTissuemicroarraysCellularm

6、icroarrays(alsocalledtransfectionmicroarraysChemicalcompoundmicroarraysAntibodymicroarraysTypesofDNAMicroarrays1.cDNAchip(DNAmicroarray,two-channelarray)cDNA芯片:–ProbecDNA(500~5,000baseslong)isimmobilizedtoasolidsurfacesuchasglass–Usingrobotspotting–TraditionallycalledDNA

7、microarray–FirstlydevelopedatStanfordUniversity2.Genechip(DNAchip,Affymetrixchip)基因芯片:–Oligonucleotide(20~80-meroligos)issynthesizedeitherinsitu(on-chip)orbyconventionalsynthesisfollowedbyon-chipimmobilization–HistoricallycalledDNAchips–DevelopedatAffymetrix,Inc.,underth

8、eGeneChip®trademark–ManycompaniesaremanufacturingoligonucleotidebasedchipsusingalternativetechnologiesH