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1、各种激发光和吸收光波长现在发现一个更全的有关激发光和发射光的波长图谱。这对CONFOCAL去做多重染色时是必不可少的参考资料。全部文件请到这里下载!ExcitationandEmissionWavelengthsofFluorophoresTavi's"FluoroTable":Commonfluorophores(fromZeissCorporationwebsite)FluorophoreAbsorptionEmissionOtherinfo1,5IAEDANS3364901,8-ANS3724804-Methylumbelliferone3855025-carboxy-2,7-
2、dichlorofluorescein5045295-Carboxyfluorescein(5-FAM)4925185-Carboxynapthofluorescein(pH10)512/598563/668RatioDye,pH5-Carboxytetramethylrhodamine(5-TAMRA)5425685-FAM(5-Carboxyfluorescein)4925185-HAT(HydroxyTryptamine)370-415520-5405-HydroxyTryptamine(HAT)370-415520-5405-ROX(carboxy-X-rhodamine)5785
3、676045915-TAMRA(5-Carboxytetramethylrhodamine)5485425525686-Carboxyrhodamine6G5185436-CR6G5185436-JOE5205487-Amino-4-methylcoumarin3514307-AminoactinomycinD(7-AAD)5466477-Hydroxy-4-methylcoumarin360449,4559-Amino-6-chloro-2-methoxyacridine412,430471,474ABQ344445AcidFuchsin540630ACMA(9-Amino-6-chlo
4、ro-2-methoxyacridine)412,430471,474AcridineOrange+DNA502526AcridineOrange+RNA460650AcridineOrange,bothDNA&RNA440-480520-650AcridineRed455-600560-680AcridineYellow470550Acriflavin436520AcriflavinFeulgenSITSA355-425460Aequorin(Photoprotein)466PhotoproteinAFPs-AutoFluorescentProtein-(QuantumBiotechno
5、logies)seesgGFP,sgBFPAlexaFluor350346342442441AlexaFluor430431540AlexaFluor488495,492519,520AlexaFluor532531,532553,554AlexaFluor546556,557572,573AlexaFluor568577,578603AlexaFluor594590,594617,618AlexaFluor633632650AlexaFluor647647666AlexaFluor660668698AlexaFluor680679702AlizarinComplexon530-560,5
6、80580,624-645AlizarinRed530-560580Allophycocyanin(APC)630,645655,660AMC,AMCA-S345445AMCA(Aminomethylcoumarin)345347425444AMCA-X353442AminoactinomycinD555655Aminocoumarin346350442445Aminomethylcoumarin(AMCA)345347425444AnilinBlue600Anthrocylstearate360-381446APC(Allophycocyanin)630,645655,660APC-Cy
7、7625-650755APTRA-BTC=RatioDye,Zn2+466/380520/530RatioDye,Zn2+APTS424505AstrazonBrilliantRed4G500585AstrazonOrangeR470540AstrazonRed6B520595AstrazonYellow7GLL450480Atabrine436490ATTO-TAG?CBQCA465560ATTO-TAG?FQ4865