Utility of one-step nucleic acid amplification (OSNA) assay in detecting breast cancer metastases of sentinel lymph nodes in a Chinese population

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1、BreastCancer(2015)22:135–140DOI10.1007/s12282-013-0461-7ORIGINALARTICLEUtilityofone-stepnucleicacidamplification(OSNA)assayindetectingbreastcancermetastasesofsentinellymphnodesinaChinesepopulationDaliLi•XiaoliXu•JiajianChen•JiayingChen•BenlongYang•WentaoYang•Weiping

2、Xu•JiongWu•DarenShiReceived:26November2012/Accepted:6March2013/Publishedonline:21March2013ÓTheJapaneseBreastCancerSociety2013Abstractof76.9%(95%CI60.3–88.3%),andanegativepredic-BackgroundTheone-stepnucleicacidamplificationtivevalueof97.8%(95%CI95.0–99.1%)basedonthe(

3、OSNA)assayisaninnovativemethodforthediagnosisofnumberofSLNssampledbeforethediscordantcasessentinellymphnode(SLN)metastasesinbreastcanceranalyses.Elevenoutof15discordantcasescanbepatients.Theaimofthepresentstudywastoclinicallyexplainedbytissueallocationbias.validate

4、theOSNAassayandcompareitsresultswithConclusionsOurstudyshowsthattheOSNAassayispostoperativeserialsectioninginaChinesebreastcancermorestandardized,objective,andreproducibleandcanpopulation.utilizemorelymphoidtissuethanthetraditionalpatho-MethodsAprospectivestudyof37

5、0consecutiveSLNslogicalexaminationmethods.OSNAcanalsodistinguishfrom115patientswasconductedatourinstitution.Atotalbetweenmicrometastasisandmacrometastasis,therebyof311SLNsunderwentOSNAassayanalysis.AllSLNsenablingustofurtherstudythesignificanceofmicrome-weresectione

6、dinapproximately2-mmpieces.OSNAtastasis.Sincethereisalackofstandardizationandassayandpostoperativeserialsectioningwereperformedreproducibilityofpathologicalexaminationanddiagnosticonalternatedividednodesamples.ThepostoperativeserialcriteriaoftheSLNs,werecommendthat

7、theOSNAassaysectioninghistologydiagnosiswasusedasthestandard.canbeusedindailyclinicaldiagnosticwork.ResultsTheoverallrateofagreementbetweenOSNAassayandthepostoperativeserialsectioningwas95.2%KeywordsBreastcancer
Sentinellymphnode
One-[95%confidenceinterval(CI)91.6–9

8、6.9%],withasen-stepnucleicacidamplification(OSNA)sitivityof83.3%(95%CI66.5–93.0%),aspecificityof96.7%(95%CI93.7–98.4%),apositivepredictivevalueIntr