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1、Wat.Res.Vol.31,No.3,pp.471478,1997©1997ElsevierScienceLtdPergamonPrintedinGreatBritain.AllrightsreservedPll:S0043-1354(96)00281-30043-1354/97$17.00+0.00ASLUDGECHARACTERIZATIONASSAYFORAEROBICANDDENITRIFYINGPHOSPHORUSREMOVINGSLUDGEA.WACHTMEISTER,T.KUBA~,M.C.M.VANLOOSD
2、RECHT@*andJ.J.HEIJNEN@DepartmentofBiochemicalEngineering,DelftUniversityofTechnology,Julianalaan67,2628BCDelft,TheNetherlandsAbstract--Itwasrecentlyrecognizedthatdenitrifyingbacteria,aswellasaerobicbacteria,contributetothebiologicalphosphorusremovalprocess.Forevalua
3、tionofthephosphorusremovalprocessitisnecessarytohavetestsenablingapropercharacterizationofthemicrobialpopulationintheactivatedsludge.Inthisstudyweevaluatedthepossibilityofanoxicphosphorusreleaseoraerobic/anoxicphosphorusuptaketestsforsludgecharacterization.Heretowee
4、mployedsludgemixturesofenrichmentculturesfromlaboratorysequencingbatchreactor(SBR)systemsoperatedunderaanaerobic-aerobic(A/O)oranaerobic-anoxic(A2)conditions.Itisshownthatinterpretationofphosphorusreleasetests,withorwithoutnitratepresent,isdifficult.Ontheotherhandac
5、omparisonofphosphorusuptakeunderaerobicandanoxicconditionsleadstoastraightforwardcharacterizationofthemicrobialpopulationinthephosphorusremovingorganisms,withrespecttotheirphosphorusremovalactivityunderaerobicandanoxicconditions.©1997ElsevierScienceLtd.Allrightsrese
6、rvedKeywords--activatedsludge,biologicalphosphorusremoval,denitrification,denitrifyingphosphorusremovingbacteria,nitrate,sludgecharacterizationINTRODUCTIONAppeldoorn,1993;Kubaetal.,1994).Figure1showsanexampleofthephosphorusreleaseactivitywithRecentlyithasbeenshownth
7、atbesidesoxygen,andwithoutnitrateinbatchtestsusingactivatednitratecanbeutilizedasanelectronacceptorforsludgeobtainedfroma(modifiedUCT-type)wastebiologicalphosphorusremovalfromwastewaterwatertreatmentplantintheNetherlands(Kubaet(Comeauetal.,1986,1987;Vlekkeetal.,1988
8、;al.,1995).OneofthemainreasonsforthereductionPokethitiyooketal.,1990;ShinetaL,1992;Wannerofphosphorusreleasebynitratemightbetheetal.,1992;
