生物膜层干涉技术及其应用

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1、生物膜层干涉（BLI）技术及其应用BiolayerInterferometry（BLI）生物分子结合后的BLIBiosensor•生物分子结合到传感器表面形成了一层生物膜•生物膜对透过传感器的光波造成干涉现象•干涉现象以相位移动的方式被检测，从而可以检测结合到传感器表面分子数量的变化PallForteBio解决方案Label-freeRealtimeFluidics-freeFast，Accurate，Easy，InformationProvidedbyOctetPlatformAffinitycharacterization•BindingKD•Onandoffrates•R

2、ankorderSpecificityofbinding•Yes/Nobindingscreens•Epitopebinning/mapping•IsotypingProteinConcentration•PicogramtomilligramdetectionrangeFortebioProductPortfolioBlitzOctetQKeOctetQK384OctetRED96OctetRED384MWRange分子量范围>10,000D>5000D>5000D>150D>150D#ChannelsperRead通道11-81-161-81-16MicroplatePositio

3、ns板位11212BiosensorReracking可回收YESYesYesYESYesRobotCompatible兼容性NoNoYesNoYesSingle96/96HA96/96HAMicroplateFormats板型96,96HA96channel384/384TW384/384TWReadCapabilities使用范围Concentration,Kon,Koff,KD(Affinity)从浓度到亲和力SampleUsage样品使用Non-destructiveandrecoverable无损害可回收TemperatureControl温控4°Caboveambientt

4、o40°C仪器外形BiosensorselectionAccordingtoapplication&sampletypeetc.2012年开发了两种传感器1.Anti-GSTBiosensor:用于含GST标签的蛋白2.NTABiosensor：用于含His标签的蛋白BLIVersatilityinInteractionAnalysisSizeBacteria细菌MW1000nmCells细胞Virus病毒200nmBacteria细菌Antibody–Antigen抗体-抗原Virus病毒75nm1,000,000Receptor–Ligand受体-配体IgM免疫球蛋白MDNA-DN

5、AIgA免疫球蛋白ADNA-Protein7nmIgG,IgD,IgE100,000AntibodyFragmentsAntibodyFragment–Antigen抗体片段-抗原抗体片段Antigen–FusionProtein融合入蛋白Proteins蛋白质Antibody–Peptide抗体-肽Peptides肽1,000MultipleAntibodyPairings多重抗体配对1nmNucleotides(DNA)150Antibody抗体-SmallMoleculeSmallMolecules小分0.1nm1子Protein蛋白-SmallMoleculeAtoms原子BL

6、ISolutionforDrugDevelopmentWideFitWithinDiscoveryandDevelopmentProcessLeadPreclinicalandManufacturingResearchScreeningCharacterizationClinicalandQCAssaydevelopmentSmallmoleculeorAffinityKineticprofilingofProductionligandscreeningmeasurementimmunotherapeuticsmonitoringExpressionmonitoringKinetics

7、creeningofOnandoff-ratesRapidconcentrationProcesshybridomasmeasurementsoptimizationCloneselectionandBindingaffinityrankingSecondaryhitrankingImmunogenicityProteinandsmallconfirmationmoleculeEpitopemapping21CFRComplianttherap