Waters - HPLC Troubleshooting Guide

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1、HPLCTroubleshootingGuideClickonsectionsbelowtogodirectlytothatsectionoftheTroubleshootingGuideHPLCTroubleshootingUweD.Neue,WatersCorporation1.ColumnLife-Time27.OptimalFlowRates2.VariableRetentionTimes28.CarbonLoad3.DriftingRetentionTimes29.pHControl4.Column-to-ColumnandBatch-to-Bat

2、ch30.MobilePhaseCompositionReproducibility31.ColumnContamination5.SamplePreparationProblems32.MethodVerification6.SourcesofPeakTailing33.DoublePeaksinSugarSeparations7.Normal-PhaseChromatography34.MethodControl8.SystemVolume,DeadVolume,DwellVolume35.MobilePhasepH9.TransferofGradien

3、tMethods36.Signal-to-NoiseImprovements10.CloggedSystem37.Overload11.ColumnPlate-Count38.ColumnDurability12.ColumnBackpressure39.FastAnalysisandColumnBackpressure13.PeakAreaFluctuations40.ColumnBackflushing14.GhostPeaks41.SelectivityShift15.DependenceofRetentionTimesonpH42.NewMethod

4、16.ColumnEquilibration43.NegativePeaks17.ColumnConditioning44.Tricky,Tedious,TimeConsuming18.ComplexSampleMatrices45.FastSeparations19.HydrophobicCollapse46.BuffersforLC/MS20.BaselineNoise47.AlkalineBuffersforRPLC21.Narrow-boreColumns48.Post-ColumnDerivatization22.SampleSolvent49.G

5、radientDwellVolume23.GradientScaling50.BufferCapacity24.ColumnStorage51.FlowRateChangesandQuantitation25.Paired-IonChromatography52.Analysisofpolarcompounds26.HydrolyticStabilityofReversed-PhasePackings121.ColumnLife-Timecartridgewithasimilarchemistryastheseparationcolumnworkswellf

6、orthisproblem.Q.:Mycolumnlastedonlyforabout100injections.AfterAnotherandmorepowerfulapproachistouseaguardthattime,thepeaksbecamedistortedandtheplate-countscolumn.Theprecolumnservesasasacrificialcolumntopwereverylow.What’swrong?thatisreplacedwhentheproblemoccurs.Forbestperformance,y

7、oushoulduseaguardcolumnthatcontainsA.:100injectionsisindeedashortlife-time.Undernormalexactlythesamepackingastheanalyticalcolumnandiscircumstances,onecanexpectacolumntobeinservicepackedwiththesamehighperformancepackingtechniqueasmuchlonger.Inordertodeterminewhatiswrong,weneedtheana

8、lyticalcolumn.Ifyouuseprec