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1、CoRRESPoNDENCErevisionprocessofthiswork,anindependentCOMPETINGFINANCIALINTERESTSfrom14.5%to20.0%,asestimatedbybandstudyreportedthesimultaneousgenerationTheauthorsdeclarenocompetingfinancialinterests.intensities(Fig.1candSupplementaryofmultiplemutationsinmice7.Ourwork,1,31–311Meth
2、ods).CloningandsequencingoftheseWeiLi,FeiTeng,TiandaLi&QiZhoutogetherwiththemicework,demonstratesuncutbandsrevealedindelsinthetargeted1StateKeyLaboratoryofReproductiveBiology,thatitshouldbefeasibletoproducegene-OsPDSgene.ThehighestfrequencyofInstituteofZoology,ChineseAcademyoftar
3、getedmodelsinrodentsandprobablymutationswasobtainedwithansgRNASciences,Beijing,China.2UniversityofChineseothermammalianspeciesusingtheCRISPR-3with20nucleotides(nts)ofsequenceAcademyofSciences,Beijing,China.TheseCassystems.complementarytotheOsPDS-SP1targetauthorscontributedequally
4、tothiswork.e-mail:qzhou@ioz.ac.cnsite(P=0.039)(SupplementaryFig.1c,d).Note:SupplementaryinformationisavailableintheWetargetedanotherthreericegenesonlineversionofthepaper(doi/10.1038/nbt.2652).1.Jinek,M.etal.Science337,816–821(2012).(OsBADH2,Os02g23823andOsMPK2)and2.Cho,S.W.,Kim,S
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