玉米耐荫性的QTL分析

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1、英文摘要QTLanalysisofmaizeshadetolerantSupervisor：Prof．LichaohaiMasterCandidate：yuanliuzhengMaizeisallimportantfood，feedandindustrialcrops，asChina’Ssecondlargestfoodcrops，perennialplantingaroundanareaofabout4millionmu，thetotaloutputofabout150milliontons，inthenationalecon

2、omyoccupiesanimportantposition．Thestressofrainandpoorlightduringthemaizegrowingisoftenencounteredinnon-biologicaladversity，isalsotheimportantissueofmaizeproductionoftheHuang。Huai-Haiareaintoday’sfacing．Eachyearduetostresscausedbylowlight．comproductionislowedby10—15％，

3、inseriouscasesupto20％．30％．Low-lightstresshasbecomeasustainabledevelopmentofChina’Smaizeproductionofthemajorconstraints．Breedingexcellenttolerantmaizehybridsofmaizegeneticsandbreedingisoneoftheimportantgoals．Toleranttomiscontrolledbymultiplegenesofquantitativetraitsus

4、ingtraditionalbreedingmethodstoimprovetheirtolerantandlessefficient．Inthisstudy，inbredlinesofstrongtolerantofthe中72andinbredlinesofweaktolerantofthe502wereforexperimentalmaterials，andtheF2mappinggroupswereprepared．Inlow-lightstressandnon．stressconditions，inZhengzhoua

5、ndxunxian，investigatetheF3familiestolerantfield．relatedphenotypictraits，thenumberofdaystoheading，silkingdays，maleandfemaleinterval，specificleafweight，stemdiameter，plantheight，earheight．TolerantQTLassociatedtothelocationandgeneticanalysisareaslodone．TolerantmaizeQTLco

6、nsistencyandinteroperabilitybetweentheeffectagainsttolerantarediscussedinthegeneticbackgroundofdifferentgroupsofmappingQTLpositioning．Themaintrialfindingswereasfollows：1716pairsofSSRprimersformaizeinbredlinesin72×502werefortheparentalpolymorphismscreening，atotalof210

7、pairsofpolymorphismswereselectedprimers，andthetagsdeviatedseriouslywereremoved．UsingmappingsoftwareMapmaker3．0，geneticlinkagemapofmaizewasobtainedwith197polymorphism，thetotallengthofmap2693．6CM，theaveragegenome．widemap13．67cM．aminimummapdistance0．5cM．2AtxunxianandZhe

8、ngzhouthetwopilotsites，F2：3familiestassellingdays，silkingdays，ASI，specificleafweight，stemdiameter，plantheight，earheight，whicha】『e河南