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《砧木对小型早熟西瓜果实糖代谢及相关酶活性的影响_刘慧英》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、园艺学报2004,31(1):47~52ActaHorticulturaeSinica砧木对小型早熟西瓜果实糖代谢及相关酶活性的影响1,21*13刘慧英朱祝军钱琼秋葛志平123(浙江大学蔬菜研究所,杭州310029;新疆石河子大学园艺系,石河子832003;嘉兴市秀洲区农林局,嘉兴314000)摘要:以小型西瓜品种小兰自根植株为对照,研究杭州长瓠、超丰F1、勇士、黑籽南瓜4种砧木对其果实糖代谢特性及相关酶活性的影响。结果表明:4种嫁接西瓜果实发育过程中蔗糖、葡萄糖、果糖含量和糖代谢酶活性变化趋势与自根西瓜一致。在果实发育早期,果实中以分解酶
2、类为主,糖分积累低;发育后期以合成酶类为主,糖分积累多。西瓜嫁接后果实糖分含量明显降低,降低程度依砧木而异,超丰F1/小兰、杭州长瓠/小兰西瓜的糖含量明显高于黑籽南瓜/小兰、勇士/小兰。砧木可能主要通过影响果实发育早期转化酶(Inv)、发育后期蔗糖磷酸合酶(SPS)、蔗糖合酶(SS)活性来影响糖分积累。各处理糖代谢酶的净活性与糖分积累存在较好相关性,说明糖代谢酶在西瓜果实糖分积累中的作用是综合的,造成自根及嫁接西瓜果实糖分积累的差异是Inv、SPS、SS几种酶共同作用的结果。关键词:西瓜;砧木;嫁接;糖含量;糖代谢酶活性中图分类号:S651文献标识码:A文章编号:05
3、13-353X(2004)01-0047-06TheEffectsofDifferentRootstocksontheSugarMetabolismandRelatedEnzymeActivitiesinSmallandEarly-maturingWatermelonduringFruitDevelopment1,2113LiuHuiying,ZhuZhujun,QianQiongqiu,andGeZhiping12(InstituteofVegetableScience,ZhejiangUniversity,Hangzhou310029,China;Departmentof
4、Horticulture,XinjiangShiheziUni-3versity,Shihezi832003,China;BureauofAgricultureandForestry,Jiaxing314000,China)Abstract:Thesugarmetabolismandtheirrelatedenzymeactivitiesduringfruitdevelopmentofown-rootedandgraftedonHangzhou-longgourd,ChaofengF1,YongshiandFigleafgourdwatermelonwerestudied.T
5、heresultsshowedthatthechangesofsugarcontentandsucrosemetabolismenzymeactivityduringthefruitdevelopmentofdif-ferentgraftedandown-rootedwatermelonweresimilar.Atanearlystageoffruitdevelopment,sugarsaccumulatedslowlyduetohigherenzymeactivityforsucrosecleavagethanforsucrosesynthesis.Atafruitripe
6、ningstage,thesugarcontentincreasedrapidlybecausetheenzymeactivityforsucrosesynthesiswashigherthanforsucrosecleav-age.However,thesugarcontentinthefruitsofallgraftedwatermelonswerelowerthanthatinthefruitofown-rootedwatermelon.Amongthegraftedwatermelonfruits,thesugarcontentofthewatermelongraft
7、edonChaofengF1andHangzhou-longgourdwashigherthanthatofwatermelongraftedonFigleafgourdandYongshi.Theactivitiesofinvertaseattheearlystage,sucrosephosphatesynthaseandsucrosesynthaseatthelatestageinfluencedbygraft-ingmayberesponsibleforthedecreaseofsugarcont
