多胺浸种对玉米抗渗透胁迫能力的影响

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1、杜红阳：多胺浸种对玉米抗渗透胁迫能力的影响EFFECTSoFPoLYAMINESEEDSORKINGONTOLERANCEOFMAIZE(ZEAMAYSL)TOOSMOTICSTRESSSupervisor：ProfesserYangQing—huaMaster(Ph．D)Candidate：DuHong-YangABSTRACT：Here，twomaizecultivars、vitlldifferentdroughtstress—tolerance(Nongda108CV．，drought-t

2、olerant；xundan20CV．，drought—sensitive)wereusedasexperimentalmaterialstostudytheeffectsofpolyamineseedsoakingOilthetoleranceofmaizetoosmotic．stressatthestageofgerminationandseedling，inordertodiscovertheproperkindandconcentrationofpolyaminewhichenhance

3、thetoleranceofmaizetoosmoticstressandprobethemechanism．Theresultsareasfollow：1EffectsofpolyamineseedsoakingongerminationofmaizeseedunderosmoticstressThegerminationrate，germinationenergyandcoleoptilelengthofXundan20seedsdecreasemoresignificantlythanNo

4、ngda108underosmoticstress．Theseindexeschangeinsignificantlywithtreatmentofdifferentconcentrationofputrescineanddecreaseunderllighconcentration．However，theseindexesofXundan20increasemoremarkedlythanthoseofNongda108withtreatmentofdifferentconcentration

5、ofspermidineandspermineandtheseindexesaremaximalat0．5mmol／Lconcentration．TheresultssuggestthatSpdandSpmseedsoakingatlowconcentrationcouldpromoteseedgerminationunderosmoticstress，butputrescinetreatmentseemsinsignificant．Themechanismwhichpolyamineseeds

6、oakingalleviatetheinhibitionofosmoticstresstomaizeseedgerminationisthatpolyamineseedsoakingatproperconcentrationenhancestheactivitiesofamylaseandproteaseandfacilitatesthehydrolysisofstoragesubstances，e．g．starch．2Effectofseedsoakingwithspermidineontol

7、eranceofmaize(ZeamaysL·)coleoptiletoosmoticstressOnthe4mday,afterpolyethyleneglycol(PEG)一6,000treatment，RELofcoleoptileofXundan20increasedmoresignificantlythanthatofNongda108，whiletheSpdcontentinlatterincreasedmoresignificantly；LengthandRWCofcoleopti

8、leofXundan20decreasedmoresignificantlythanthatofNongda108．Underosmoticstress，treatmentofseed-soakingwithSpdresultedintheincreasesinlength，RWCandSpd，inconcertwithandecreaseinRELofcoleoptileofXundan20．TheresultssuggestedthatSpdseed·soakingcouldenhanceg