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ID:39129447
大小:1.87 MB
页数:44页
时间:2019-06-25
《多胺浸种对玉米抗渗透胁迫能力的影响》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、杜红阳:多胺浸种对玉米抗渗透胁迫能力的影响EFFECTSoFPoLYAMINESEEDSORKINGONTOLERANCEOFMAIZE(ZEAMAYSL)TOOSMOTICSTRESSSupervisor:ProfesserYangQing—huaMaster(Ph.D)Candidate:DuHong-YangABSTRACT:Here,twomaizecultivars、vitlldifferentdroughtstress—tolerance(Nongda108CV.,drought-t
2、olerant;xundan20CV.,drought—sensitive)wereusedasexperimentalmaterialstostudytheeffectsofpolyamineseedsoakingOilthetoleranceofmaizetoosmotic.stressatthestageofgerminationandseedling,inordertodiscovertheproperkindandconcentrationofpolyaminewhichenhance
3、thetoleranceofmaizetoosmoticstressandprobethemechanism.Theresultsareasfollow:1EffectsofpolyamineseedsoakingongerminationofmaizeseedunderosmoticstressThegerminationrate,germinationenergyandcoleoptilelengthofXundan20seedsdecreasemoresignificantlythanNo
4、ngda108underosmoticstress.Theseindexeschangeinsignificantlywithtreatmentofdifferentconcentrationofputrescineanddecreaseunderllighconcentration.However,theseindexesofXundan20increasemoremarkedlythanthoseofNongda108withtreatmentofdifferentconcentration
5、ofspermidineandspermineandtheseindexesaremaximalat0.5mmol/Lconcentration.TheresultssuggestthatSpdandSpmseedsoakingatlowconcentrationcouldpromoteseedgerminationunderosmoticstress,butputrescinetreatmentseemsinsignificant.Themechanismwhichpolyamineseeds
6、oakingalleviatetheinhibitionofosmoticstresstomaizeseedgerminationisthatpolyamineseedsoakingatproperconcentrationenhancestheactivitiesofamylaseandproteaseandfacilitatesthehydrolysisofstoragesubstances,e.g.starch.2Effectofseedsoakingwithspermidineontol
7、eranceofmaize(ZeamaysL·)coleoptiletoosmoticstressOnthe4mday,afterpolyethyleneglycol(PEG)一6,000treatment,RELofcoleoptileofXundan20increasedmoresignificantlythanthatofNongda108,whiletheSpdcontentinlatterincreasedmoresignificantly;LengthandRWCofcoleopti
8、leofXundan20decreasedmoresignificantlythanthatofNongda108.Underosmoticstress,treatmentofseed-soakingwithSpdresultedintheincreasesinlength,RWCandSpd,inconcertwithandecreaseinRELofcoleoptileofXundan20.TheresultssuggestedthatSpdseed·soakingcouldenhanceg
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