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时间:2017-11-25
《低氧化还原电位对铜绿微囊藻(microcystis aeruginosa)生长的抑制及恢复的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、t,.LakeSci.(湖泊科学),2012,24(4):528.534http:#www.jlakes.org.E—mail:jlakes@nighs.ac.cn◎2012byJournalofLakeSciences低氧化还原电位对铜绿微囊藻(Microcystisaeruginosa)生长的抑制及恢复的影响王健,周天艺H,高文程,浦海清,杨柳燕“(1:南京大学环境学院,南京210046)(2:污染控制与资源化研究国家重点实验室,南京210046)摘要:在实验室光照黑暗交替培养下,用半胱氨酸下调黑暗起始时的氧化还原电位(OR
2、P),研究低电位对铜绿微囊藻(MicrocystisaeruginosaFACHB469)生长的影响,并探索低电位胁迫消除后铜绿微囊藻生长的恢复潜力.结果表明,在一50一一100mV范围内水体ORP越低,其对铜绿微囊藻生长的抑制作用越强.低ORP可抑制铜绿微囊藻对磷的吸收,且这种抑制程度与电位变化无关.一5O~一75mV的电位对铜绿微囊藻SOD酶活性有促进作用,而一9O~一100mV电位明显抑制SOD酶活.当低ORP胁迫消除后,铜绿微囊藻能够恢复至最初的生长状态,同时伴随含磷量的提高和SOD酶活的恢复,一5O一一75mV电位胁迫
3、对藻消除胁迫后的生长更有促进作用.因此,低ORP对铜绿微囊藻的生长会产生不利影响,但是该影响是可逆的.关键词:铜绿微囊藻;氧化还原电位;生长抑制;恢复;磷;SOD酶;叶绿素aGrowthinhibitionandrecoveryfr0mlowoxidationreductioninosaWANGJian,ZHOUTianyi,GAOWencheng,PUHaiqing&YANGLiuyan,(1:SchooloftheEnvironment,NanjingUniversity,Nanjing210046,P.R.China)(2
4、:StateKeyLaboratoryofPollutionControlandResourceReuse,Nanjing210046,P.R.China)Abstract:Inthisexperiment,cysteinewasusedasanOxidationReductionPotential(ORP)adjustmenttoexploretheresponsesofMicrocystisaeruginosa(FACHB469)underdiferentORPs’stimulation.Arecoveryexperimen
5、twasalsodesignedwithaeruginosaseparatedfromtIleformerexperimentandculturedinfreshmedium.ResultsshowedanegativeeffectoflowORPstimu—lationonthegrowthofaeruginosa.Withintherangeof一50to一100mV.inhibitionefectwasintensifledwiththeORP’sde—cline.Theuptakerateofphosphorusdecr
6、easedunderlowORP’sstressbuthadnorelationshipwithstressstrength.AproperlowORP’sstimulation(e.g.一50and一75mY)couldintensifytheSODenzymeactivityofM.aeruginosa,whileoverlow(e.g.一90and一100mV1hadnegativeefects.aeruginosacouldrecoveritsgrowingabilityafterremovingthelowerORP’
7、sstimu—lation.TheuptakerateofphosphorusandtheSODenzymeactivityreturnedtonormalaswel1.Moreover,theenhancementofgrowthabilitywasobservedbyalowORP’sstimulation(e.g.一50and一75mV).Thus,thelowORP’sstimulationhadnega—tireeffectonthegrowthofM.aentginosa,howevertheprocesscanhe
8、reversible.Keywords:Microcystisaeruginosa;oxidationreductionpotential;growthlimit;recovery;phosphorus;SODenzyme;chlorophyl—a湖泊富营养化问
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