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ID:39077186
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页数:8页
时间:2019-06-24
《A microfluidic model of the blood–brain barrier to study permeabilization by pulsed electric fields英文资料》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、RSCAdvancesViewArticleOnlinePAPERViewJournal
2、ViewIssueAmicrofluidicmodeloftheblood–brainbarriertostudypermeabilizationbypulsedelectricfields†Citethis:RSCAdv.,2017,7,42811M.Bonakdar,*P.M.GraybillandR.V.DavalosPulsedelectricfieldsinteractwiththeblood–brainbarrier(
3、BBB)andhavebeenshowntoincreasetheBBBpermeabilityundersomepulsingregimes.PulsedelectricfieldsmayenhancedrugdeliverytothebrainbydisruptingtheintegrityoftheBBBandallowingotherwiseimpermeabledrugstoreachtargetareas.Microfluidic,invitromodelsofferanalternativeplatfor
4、mforexploringtheimpactofpulsedelectricfieldsontheBBBbecausetheycreatephysiologicallyrelevantmicroenvironmentsandeliminatetheconfoundingvariablesofanimalstudies.Wedevelopedamicrofluidicplatformforreal-timemeasurementofBBBpermeabilitypre-andpost-treatmentwithpuls
5、edelectricfields.Permeabilityismeasuredopticallybythediffusionoffluorescenttracersacrossamonolayerofhumancerebralmicrocapillaryendothelialcells(hCMECs)culturedonapermeablemembrane.WefoundthatthisdeviceReceived10thJuly2017isabletocapturereal-timepermeabilityofhCM
6、ECmonolayersforbothreversibleandirreversibleCreativeCommonsAttribution-NonCommercial3.0UnportedLicence.Accepted26thAugust2017electroporationpulsingregimes.Furthermore,preliminarytestingofdeepbrainstimulationpulsingDOI:10.1039/c7ra07603gregimesrevealspossiblei
7、mpactsonBBBintegrity.Thisdevicewillenablefuturestudiesofpulsedrsc.li/rsc-advanceselectricfieldregimesforimprovedunderstandingofBBBpermeabilization.Introductionbyhamperingdrugtransporttothebrain.TherearetwomajorpathwaysforthetransferofsubstancesacrosstheBBBwhic
8、harecategorizedasparacellularandtranscellular.5TransportCerebralmicrovesselsstrictlyregulatethetransferofsubstancesbetweenthebloodandthebraintissue.Thisregu-acrosstheintercellularTJs,theparacellularpathway,restrictsThisarticleislicensedunderalationbycerebralm
9、icro-vesselsiscalledtheblood–brainbarriertransporttosmallhydrophilicmoleculesoflessthan500Dainmolecularweight.5Transportthroughthecellmembraneand(BBB),andisduetointercellulartightjunction
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