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1、JournalofAppliedMicrobiologyISSN1364-5072ORIGINALARTICLEDevelopmentofparatransgenicArtemiaasaplatformforcontrolofinfectiousdiseasesinshrimpmariculture111231B.Subhadra,I.Hurwitz,A.Fieck,D.V.S.Rao,G.SubbaRaoandR.Durvasula1DepartmentofInternalMedicine,Schoo
2、lofMedicine,UniversityofNewMexico,Albuquerque,NM,USA2EmeritusScientist,BedfordInstituteofOceanography,Dartmouth,NS,Canada3ShreeVasudhaLaboratories,Visakhapatnam,IndiaKeywordsAbstractArtemia,diseasecontrol,paratransgenesis,shrimpculture,shrimppathogens,Ai
3、m:TostudytheaccumulationandretentionofrecombinantproteinsinSynechococcusbacillarus.Artemiagutforoptimizingparatransgenicdiseasecontrolinshrimpaquaculture.MethodsandResults:TransgenicEscherichiacoliexpressingfluorescentmarkerCorrespondenceproteinsandthetra
4、nsgeniccyanobacteriumSynechococcusbacillarusexpressingRaviDurvasula,DepartmentofInternalafunctionalmurinesinglechainantibody,DB3,werefedtoArtemiafrancis-Medicine,MSC55510,1UniversityofNewcana.Stableexpressionandretentionofseveralmarkermolecules(e.g.GFP,M
5、exico,Albuquerque87131,NM,USA.DSRedandDB3)upto10hafteroffeedingwithE.coliwereevidentwithinE-mail:Ravi.Durvasula@med.va.govthegutofArtemia.EngineeredstrainsofS.bacillarusexpressingDB3accumu-20081723:received8October2008,revisedlatedwithinthegutofArtemiawi
6、thdetectableantibodyactivityfor8–10hof29June2009andaccepted30June2009feedingviaELISA,coincidentwiththetimeperiodofthehighestdensityoftransgenicS.bacillarusintheArtemiagut.doi:10.1111/j.1365-2672.2009.04479.xConclusions:Artemiafedtransgenicbacteriaoralgae
7、accumulatedrecombinantproteinsforupto10hthatretainedbiologicalactivity.Co-deliveryofmultiplerecombinantproteinssimultaneouslyinthegutofArtemiawasalsodemon-strated.SignificanceandImpactoftheStudy:Expressionofmoleculesthattargetinfectiousagentsofmariculture
8、inshrimpviacommonlydeployedfeedorgan-ismssuchasArtemiacouldpotentiallyofferpowerfulnewtoolsintheongoingglobalefforttoincreasefoodsupply.3weekspostvaccination(Witteveldtetal.2004;JohnsonIntroductionetal.2008).Othernovelappr
