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1、AquacultureResearch,2013,16doi:10.1111/are.12225SHORTCOMMUNICATIONMolecularcloningandcharacterizationofanovelpathogen-inducedtrypsin-likeproteaseinScyllaparamamosain(Estampador1949)JianDing,HuiPeng,QianCui&Ke-JianWangStateKeyLaboratoryofMarineEnvironmentalSci
2、ence,Xiamen,Fujian,ChinaCorrespondence:K-JWang,StateKeyLaboratoryofMarineEnvironmentalScience,Xiamen,Fujian,China.E-mail:wkjian@xmu.edu.cn.Serineprotease(SP)isoneoftheoldestcharacter-keepitundertightcontrolbyanotherproteinase,izedandlargestmultigeneproteolyti
3、cfamilies,intheircofactors(non-catalyticserineproteinasewhichserineservesasthenucleophilicaminoacidhomologues)andserineproteinaseinhibitors.Allatthecatalyticsite.Theactiveserineandtwothoseproteinasescanproperlybereferredtoasotherresidues(ahistidineandanaspart
4、ate)areprophenoloxidase-activatingfactors(PPAF)(Budareferredtoasthecatalytictriadinthecatalytic&Shafer2005).ManyPPAEgenesencodingeithersitesofmanyfamiliesofSPincludingtrypsin(S1),serineproteases(SPs)orserineproteasehomologsalpha-lyticendopeptidases(S2),togavi
5、rusendopep-(SPHs)havebeenclonedandidentifiedfromavari-tidase(S3),subtilisn(S8),prolyloligopeptidaseetyofarthropodspeciessuchasHolotrichiadiompha-(S9)andserinecarboxypeptidase(S10)familieslia(Kwon,Kim,Choi,Joo,Cho&Lee2000;Kim,(Rawlings&Barrett1993).Inthetrypsin
6、subfam-Baek,Lee,Park,Lee,Soderh€all&Lee2002),€Mand-ily,thethreeresidues,serine,histidineandaspar-ucasexta(Jiang,Wang&Kanost1998;Yu,Jiang,tateare,respectively,surroundedbyGDSGGP,Wang&Kanost2003;Gupta,Wang&JiangTAAHCandDIMLL,whichareallhighlycon-2005),thecrayfis
7、hPacifastacusleniusculus(Wang,servedmotifs(Yousef,Elliott,Kopolovic,Serry&Jiang&Kanost2001),theblacktigershrimpDiamandis2004).SerineproteasehomologsPenaeusmonodon(Amparyup,Jitvaropas,Pulsook&(SPHs)aresimilarinaminoacidsequencetoS1Tassanakajon2007;Charoensapsr
8、i,Amparyup,familySPs,butapparentlylackamidaseactivityHirono,Aoki&Tassanakajon2011),Chinesemittenbecausethemutationofoneormoreofthecata-crabEriocheirsinensis(Gai,Qiu,Wang,Song,Mu,lyticresi
