DB35T 1218-2011 马铃薯晚疫病菌生理小种鉴定技术规程

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1、ICS65.020B16DB35福建省地方标准DB35/T1218—2011马铃薯晚疫病菌生理小种鉴定技术规程Technicalstandardforidentificationonthephysiologicalracesofphytophthorainfestansonpotato2011-12-31发布2012-03-15实施福建省质量技术监督局发布DB35/T1218—2011目次前言............................................................

2、....................II1范围..............................................................................12术语和定义........................................................................13接种体的制备....................................................................

3、..13.1培养基的配制..................................................................23.1.1黑麦培养基................................................................23.1.2选择性培养基..............................................................23.1.3MS培养基....................

4、..............................................23.2晚疫病样的采集................................................................23.3晚疫病菌的分离................................................................23.3.1保湿培养分离法.................................................

5、...........23.3.2薯片夹叶分离法............................................................23.4晚疫病菌的鉴定与纯化..........................................................23.5晚疫病菌的保存................................................................23.6孢子囊悬浮液的制备..............

6、..............................................24鉴别寄主的保存与扩繁..............................................................34.1保存..........................................................................34.2扩繁....................................................

7、......................35接种鉴定..........................................................................35.1接种方法......................................................................35.2接种技术......................................................................

8、35.3接种后的培养..................................................................36病情调查..........................................................................36.1调查时间..............................