《分子生物学》试卷一

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1、南华大学生命科学与技术学院《分子生物学》试卷一FinalexamofMolecularBiologyCourse年级______专业________学号_________姓名_______成绩______PARTI:DESCRIPTION(2pointseach)Youranswershoulddescribewhateachitemisandhowitfunctionsinthecell.Diagrams,structureandsequenceinformationshouldbeincludedinyouranswer,asnecessary.1.Yeastartific

2、ialchromosome　　酵母人工染色体　　RNAinterference　RNA干扰2.Proteomics蛋白质组学3.Shine-Dalgarnosequence服务，达尔加诺序列4.Alternativesplicing剪接5.Ribozyme核酶6.r-dependenttermination依赖终止7.RNAeditingRNA编辑8.DNAlesionsDNA损伤9.Proteintargeting蛋白定位PARTII:MULTIPLECHOICES(1pointseach)Selecttheonebestanswerforeachquestion.1.The

3、catalyticactivityforpeptidebondformation(thepeptidyltransferaseactivity)islocatedinthe:1)RNAofthelargeribosomalsubunit.2)leadersequenceofthemessengerRNA.3)RNAofthesmallribosomalsubunit.4)proteinsofthesmallribosomalsubunit.5)proteinsofthelargeribosomalsubunit.2.Bidirectionalandsemi-conservati

4、vearetwotermsthatreferto:1)transcription.2)translation.3)replication.4)alloftheabove.5)noneoftheabove.3.Thefactthatmostaminoacidsarespecifiedbymultiplecodonsisknownas:1)the“wobble”phenomenon.2)theuniversalityofthegeneticcode.3)codonbias.4)theanticodonhypothesis.5)theredundancyofthegeneticcod

5、e.4.RNApolymeraseIistheeukaryoticenzymeresponsiblefor:1)transcriptionofribosomalRNA.2)transcriptionoftransferRNAandothersmallRNAspecies.3)transcriptionofmessengerRNA.4)initiationofOkazakifragmentsynthesisinDNAreplication.5.RestrictionenzymescancleaveDNAthatiseithersingle-strandedordouble-str

6、anded,aslongasitcontainstheappropriaterecognitionsite.1)True2)False6.Informationaboutthesequenceofthecodingregionofageneisbestobtainedfrom:1)aYACclone.2)agenomicclone.3)acDNAclone.4)theprotein.7.Achromatographymethodthatcanbeusedspecificallytopurifyproteinsbasedontheirchargeis:1)gelfiltratio

7、nchromatography.2)ion-exchangechromatography.3)DNAaffinitychromatography.4)antibodyaffinitychromatography.8.AnonsensemutationisachangeintheDNAsequencethatresultsin:1)asmalldeletionorinsertion.2)anaminoacidchangeintheproteinencodedbythegene.3)aprema