2.2.27. Thin-layer chromatography(中英)

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1、2.2.27.Thin-layerchromatography薄层色谱法Thin-layerchromatographyisaseparationtechniqueinwhichastationaryphaseconsistingofanappropriatematerialisspreadinauniformthinlayeronasupport(plate)ofglass,metalorplastic.Solutionsofanalytesaredepositedontheplatepriortodevelopme

2、nt.Theseparationisbasedonadsorption,partition,ion-exchangeoroncombinationsofthesemechanismsandiscarriedoutbymigration(development)ofsolutes(solutionsofanalytes)inasolventorasuitablemixtureofsolvents(mobilephase)throughthethin-layer(stationaryphase).薄层色谱法是一种分离技术，

3、这种技术采用由适当物质作为固定相均匀涂布在玻璃、金属或塑料的支持物上。在展开前，供试品液点样在薄层板上。分离原理是供试品溶解在溶剂中或合适的混合溶剂中（流动相）在薄层上（固定相）通过吸附、分配、离子交换或以上机制共同作用移动（展开）来实现的。APPARATUS仪器Plates.Thechromatographyiscarriedoutusingpre-coatedplatesasdescribedunderReagents(Error!Nobookmarknamegiven.).薄层板色谱法是按照4.1.1试剂项下描述

4、的涂层板来进行的。Pre-treatmentoftheplates.Itmaybenecessarytowashtheplatespriortoseparation.Thiscanbedonebymigrationofanappropriatesolvent.Theplatesmayalsobeimpregnatedbyproceduressuchasdevelopment,immersionorspraying.Atthetimeofuse,theplatesmaybeactivated,ifnecessary,by

5、heatinginanovenat120°Cfor20min.薄板预处理：在分离前用适当的溶剂来清洗薄板。可用适当的溶剂洗脱。在进行展开、浸渍或喷雾显色过程中要将薄板浸透。使用时，如果需要，要在120℃干燥箱中加热20min活化薄板。Chromatographictankwithaflatbottomortwintrough,ofinert,transparentmaterial,ofasizesuitablefortheplatesusedandprovidedwithatightlyfittinglid.Forho

6、rizontaldevelopmentthetankisprovidedwithatroughforthemobilephaseanditadditionallycontainsadevicefordirectingthemobilephasetothestationaryphase.层析缸：使用适合薄层板大小的无活性、透明物质制成的层析缸，并有严密的盖子，底部平整，或有双槽。水平展开的层析缸有一个装展开剂的槽，并且额外有一个装置用来引导展开剂到固定相。Micropipettes,microsyringes,calib

7、rateddisposablecapillariesorotherapplicationdevicessuitablefortheproperapplicationofthesolutions.微量加液器、微量注射器、有刻度的一次性毛细管：或其它适合于应用溶液的装置。Fluorescencedetectiondevicetomeasuredirectfluorescenceortheinhibitionoffluorescence.荧光检测装置：用来测量直接荧光或荧光抑制Visualisationdevicesandr

8、eagents.Suitabledevicesareusedforderivatisationtotransfertotheplatereagentsbyspraying,immersionorexposuretovapourand,whereapplicable,tofacilitateheatingforvisualisati