分子生物学 常用引物序列

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1、日常备库引物序列(5'-3')1492RGGTTACCTTGTTACGACTT27F8FAGAGTTTGATCCTGGCTCA35SGACGCACAATCCCACTATCC3'ADAGATGGTGCACGATGCACAG3'AOXAOX1revGGCAAATGGCATTCTGACAT3'BDTAAGAGTCACTTTAAAATTTGTATAC5'ADGAL4ADP17110TACCACTACAATGGATGATG5'AOXAOX1forGACTGGTTCCAATTGACAAGC5'BDGAL4-BD-CforTCA

2、TCGGAAGAGAGTAG96gIIIM13-96CCCTCATAGTTAGCGTAACGa-FACTORAlphaforTACTATTGCCAGCATTGCTGCBAC1AACCATCTCGCAAATAAATABAC2ACGCACAGAATCTAGCGCTTBGHpCDNA3.1RTAGAAGGCACAGTCGAGGCMV-24TTAGGACAAGGCTGGTGGCMV-30ATAACCCCGCCCCGTTGCMV-FCMV-ProforCGCAAATGGGCGGTAGGCGTGATGGGCGGTAGGCGTGC

3、MV-RTCGTTGGGCGGTCAGCDuetDOWN1GATTATGCGGCCGTGTACAADuetUP1GATCTCGACGCTCTCCCTDuetUP2TTGTACACGGCCGCATAATCEBVrevGTGGTTTGTCCAAACTCATCEGFP-CforAGCACCCAGTCCGCCCTGAGCEGFP-NrevCGTCGCCGTCCAGCTCGAL1-ProforAACATTTTCGGTTTGTATTACTTCGLP1TGTATCTTATGGTACTGTAACTGGLP2CTTTATGTTTTTGGCGTC

4、TTCCAGLP4GCCCTTCTTAATGTTTTTGH1-FH1TCGCTATGTGTTCTGGGAAAHSVtk-pArevGTCTCCTTCCGTGTTTCAGIRES-RCCTCACATTGCCAAAAGACGITS4TCCTCCGCTTATTGATATGCITS5GGAAGTAAAAGTCGTAACAAGGM13-47CGCCAGGGTTTTCCCAGTCACGACM13FTrxReverseM13-20M13F-40TGTAAAACGACGGCCAGTM13RCAGGAAACAGCTATGACCmalEG

5、GTCGTCAGACTGTCGATGAAGCCMT-ProforCATCTCAGTGCAACTAAAmU6F2（mouse）TAGTATCCGACGCCGCCATCNL1GCATATCAATAAGCGGAGGAAAAGNL4GGTCCGTGTTTCAAGACGGNOSR-primerCGTCCAGCTCCATGTTGCP1CCAGGCTTTACACTTTATGCp10-ProforCGGACCTTTAATTCAACCCP2GCGATTAAGTTGGGTAACGCpBABE3'ACCCTAACTGACACACATTCCPBABE

6、5'CTTTATCCAGCCCTCACpBADfwCCATAGCATTTTTATCCATAAGpBADrvpTrcHis-rvATCTGTATCAGGCTGAAAATCPBV220FAAGAAGGGCAGCATTCAAAGpCDNA3.1FCTAGAGAACCCACTGCTTACpCMV5FTTCCAAAATGTCGTAATAACpCMV5RATTATAGAGGACACCTAGTCPCMV-FTCTAAAAGCTGCGGAATTGTPCMV-RTCCAAACTCATCAATGTATCpcoldI-RpCold-SUMO-R

7、GGCAGGGATCTTAGATTCTGpCold-TF-F1CCACTTTCAACGAGCTGATGpDBLeu-FGAATAAGTGCGACATCATCATCpDEST22-FTCGATGATGAAGATACCCCACCpDEST22-RCTCGACGTCTTACTTACTTAGCPDONR-FTCGCGTTAACGCTAGCATGGATCTCPDONR-RGTAACATCAGAGATTTTGAGACACpDsRED-ex-C1-FTCCCACAACGAGGACTACACPEF-FEF-1aForwardTCAAGCCT

8、CAGACAGTGGTTCpEGFP-C-3'TATGGCTGATTATGATCAGTpEGFP-C-5’CATGGTCCTGCTGGAGTTCGTGpEGFP-N-3’EGFP-NrevCGTCGCCGTCCAGCTCGACCAGpEGFP-N-5'TGGGAGGTCTA