Invitrogen中国测序通用引物序列

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1、Invitrogen中国测序通用引物序列引物名称序列(5'-3')M13RCAGGAAACAGCTATGACCM13FTGTAAAACGACGGCCAGTM13F(-47)CGCCAGGGTTTTCCCAGTCACGACM13R(-48)AGCGGATAACAATTTCACACAGGAM13(-96)CCCTCATAGTTAGCGTAACGSP6ATTTAGGTGACACTATAGT7TAATACGACTCACTATAGGGT7terminatorTGCTAGTTATTGCTCAGCGGT3ATTAACCCTCACTAAAGGGApGE

2、X-4T-5'GGGCTGGCAAGCCACGTTTGGTGpGEX-4T-3'CCGGGAGCTGCATGTGTCAGAGGGLp1TGTATCTTATGGTACTGTAACTGGLp2CTTTATGTTTTTGGCGTCTTCCARVp3CTAGCAAAATAGGCTGTCCCRVp4GACGATAGTCATGCCCCGCGpcDNA3.1RTAGAAGGCACAGTCGAGGPinPointprimerCGTGACGCGGTGCAGGGCGpCMV-FTCTAAAAGCTGCGGAATTGTpCMV-RTCCAAACTCATCAA

3、TGTATCpTRC99C-F:TTGCGCCGACATCATAACpTRC99C-R:CTGCGTTCTGATTTAATCTGpCEP-F:AGAGCTCGTTTAGTGAACCGEBV-R:GTGGTTTGTCCAAACTCATCpIRES2-EGFP.P5’:GTAGGCGTGTACGGTGGGAGpIRES2-EGFP.P3’:AACGCACACCGGCCTTATTC3'AD:AGATGGTGCACGATGCACAGCMV-FCGCAAATGGGCGGTAGGCGTGS1CAACGTGAAAAAATTATTATTCGCS6GTA

4、AATGAATTTTCTGTAGTAGG5`AOX1GACTGGTTCCAATTGACAAGC3`AOX1GCAAATGGCATTCTGACATCCα-FactorTACTATTGCCAGCATTGCTGCGAL4ADTACCACTACAATGGATGpACT2-RGTGCACGATGCACAGTTGAApB42ADF:CCAGCCTCTTGCTGAGTGGAGATGpB42ADR:AAGCCGACAACCTTGATTGGAGpEGFP-N-5’:TGGGAGGTCTATATAAGCAGAGpEGFP-N-3’:CGTCGCCGTCCA

5、GCTCGACCAGpEGFP-C-5’:CATGGTCCTGCTGGAGTTCGTGpEGFP-C-3':TATGGCTGATTATGATCAGTPBV220F:AAGAAGGGCAGCATTCAAAGPBV220R:CTGCGTTCTGATTTAATCTGU6ATGGACTATCATATGCTTACCGTA2.0revprimer:AGGCGATTAAGTTGGGTA3.0rev:GAGTTAGCTCACTCATTAGGCS.tagGAACGCCAGCACATGGAC5'TriplEx2CTCCGAGATCTGGACGAGCRVP4

6、GACGATAGTCATGCCCCGCGRVP3CTAGCAAAATAGGCTGTCCCpQE30-RGTTCTGAGGTCATTACTGGpQE30-FTGAGCGGATAACAATTTCACpEF-FTCAAGCCTCAGACAGTGGTTCpDSRED-N1-RTGAAGCGCATGAACTCCTTGpDsRED-express-C1-FTCCCACAACGAGGACTACACpCMV5RATTATAGAGGACACCTAGTCpCMV5FTTCCAAAATGTCGTAATAACP5'TGCGTACTGCGGTGATCAACP3'

7、CTGCAAGGCGATTAAGTTGGBAC2ACGCACAGAATCTAGCGCTTBAC1AACCATCTCGCAAATAAATA3'BD:TAAGAGTCACTTTAAAATTTGTATACpTarget.FCCAGGATTTTCCCAGTCACpTarget.RGGCTTTACACTTTATGCTTCT7(17base)ACATCCACTTTGCCTTTCTCpYes2.RTCGGTTAGAGCGGATGTGGAL4BDTCATCGGAAGAGAGTAGpAS2-1.RAAGAGTTACTCAAGAACAAGAApFlag-C

8、MV-F:GGTAGGCGTGTACGGTGGpFlag-CMV-R:GCACTGGAGTGGCAACTTpIRES-F:CTTACTGACATCCACTTTGCpIRES.R:CACTGCATTCTAGT