资源描述：
《烟草内生细菌Itb57的鉴定及其对烟草黑胫病的防治效果》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、第37卷第2期植物保护学报Vo.l37No.22010年4月ACTAPHYTOPHYLACICASINICAApr.2010烟草内生细菌Itb57的鉴定及其对烟草黑胫病的防治效果11,21*11马冠华周常勇肖崇刚陈国康易龙(1.西南大学植物保护学院,重庆400716;2.中国农业科学院柑桔研究所,重庆400712)摘要:Itb57是一株分离自健康烟草植株的内生细菌,室内测定表明,该菌株对烟草黑胫病菌Phytophthoranicotianae具有很强的拮抗作用。为了进一步明确其分类地位和生物防治潜力,结合形态、生理生化特性及16SrDNA序列(GenBank登
2、录号GQ153538)比对分析,鉴定Itb57菌株为枯草芽孢杆菌Bacillussubtilis;浸种和灌根接种测定结果显示,Itb57可定殖在烟草植株体内,且在不同部4位的含菌量不同,以根部最高(3.7210cfu/gFW),茎部次之,叶片中最少;Itb57发酵液对烟草黑胫病的温室防治效果为69.28%;2007和2008年的田间小区防治效果分别为61.25%和72.49%,与对照化学药剂58%甲霜锰锌可湿性粉剂的防治效果无显著差异(P>0.05)。关键词:内生细菌;烟草黑胫病菌;枯草芽孢杆菌;病害生物防治Identificationoftheendophyticbacterialis
3、olateItb57fromtobaccoplantanditscontrolefficacyontobaccoblackshankcausedbyPhytophthoranicotianae11,21*11MaGuanhuaZhouChangyongXiaoChonggangChenGuokangYiLong(1.CollegeofPlantProtection,SouthwestUniversity,Chongqing400716,China;2.CitrusResearchInstitute,ChineseAcademyofAgriculturalSciences,Chong
4、qing400712,China)Abstract:TheendophyticbacterialisolateItb57wasobtainedfromhealthytobaccoplantsanditwasshowedantagonisticeffectagainstPhytophthoranicotianaecausingtobaccoblackshankbyinvitrotests.Inordertodetermineitstaxaandpotentialapplicationinbiologicalcontro,lItb57wasidentifiedanditsefficacytoc
5、ontroltobaccoblackshankwasevaluatedinthisstudy.Basedonmorphologica,lphysiologicalandbiochemicalcharacteristics,and16SrDNA(GenBankaccessionGQ153538)phylogeneticanalysis,Itb57wasidentifiedasBacillussubtilis.Aftertobaccoplantswereinoculatedbyseedsoakingandrootirrigationwiththebacterialsuspension,
6、Itb57wasfoundtocolonizetheinteriortissuesoftobacco4plants.Thehighestamount(3.7210cfu/gfreshweight)oftheinoculatedbacteriumwasdetectedintherootswhilethelowestintheleavesoftheplants.GreenhousepotgrowntobaccoplantassaysshowedthatthecontrolefficacyofblackshankbyItb57was69.28%whenthebacteriumwasappli
7、edbyrootirrigationinadvanceofP.nicotianaeinoculation.Further,fieldtrialsin2007and2008inYouyangCountyofChongqingdemonstratedthattheapplicationofItb57controlledthediseaseby61.25%and72.49%,respectively.Statistical
