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《Nature 1997 389 349Histone acetylation in chromatin structure and transcription》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、progressHistoneacetylationinchromatinstructureandtranscriptionMichaelGrunstein....................................................................................................................................................................................
2、....................................................................Theaminoterminiofhistonesextendfromthenucleosomalcoreandaremodi®edbyacetyltransferasesanddeacetylasesduringthecellcycle.Theseacetylationpatternsmaydirecthistoneassemblyandhelpregulatetheunfo
3、ldingandactivityofgenes.Nucleosomes,whichfoldchromosomalDNA,containtwomol-NucleosomeassemblyeculeseachofthecorehistonesH2A,H2B,H3andH4.AlmosttwoInnucleosomeassembly,bindingoftheH3/H4tetramertoDNAisturnsofDNAarewrappedaroundthisoctamericcore,whichfollowedbyas
4、semblyofH2A/H2Bdimers.Newlysynthesizedrepressestranscriptioninvivoandinvitro.ButdespiteitssimilarityhistoneH4isdiacetylatedatK5/K12inorganismsasdiverseastoabeadonastring,thisbuildingblockofthechromosomeisTetrahymena,¯iesandhumans.NewlysynthesizedH3isalso12,1
5、3unexpectedlydynamic.Thehistoneaminoterminiextendfromtheacetylated,albeitinalessconservedmanner.Howimportantcore,wheretheycanbemodi®edpost-translationallybyacetylation,isthisacetylationinhistoneassembly?Chromatinassemblyfactor1phosphorylationandmethylation,a
6、ffectingtheirchargeandfunc-(CAF1)isacomplexofproteinsp150,p60andp48fromhumantion.Ofsuchmodi®cations,acetylationanddeacetylationhavecellswhichassemblesnewlysynthesizedH3andH4ontoreplicating14generatedmostinterestsincegeneactivitywas®rstcorrelatedwithDNAinvitr
7、o.Thecomplexislesscapableofassemblinghistones1histoneacetylation.Furtherindicationsofthiscorrelationemergedisolatedfromnuclei,suggestingthatthedifferentacetylationpatternwhenanantibodyrecognizinge-N-acetyllysinewasshowntobindofchromosomalhistonesprecludesass
8、emblyinvitro.CAF1canbindchromatinenrichedinaglobingenefromchickenerythrocytes.H4thatismono-,di-ortriacetylatedatK5,K8andK12,oreven2Bindingcontinuedevenwhenthegenewasinactive,indicatingthatunacet
