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1、《癌症》ChineseJournalofCancer,2006,25(10):1247-12511247·基础研究·二烯丙基三硫通过Caspase-3途径诱导人胃癌MGC803细胞凋亡肖晓岚,彭军,苏琦,向姝霖,唐国华,黄幼生,周秀田DiallylTrisulfideInducesApoptosisofHumanGastricCancerCellLineMGC803ThroughCaspase-3PathwayXIAOXiao-Lan,PENGJun,SUQi,XIANGShu-Lin,TANGGuo-Hua,HUANGYou-Sheng,ZHOUXiu-Tian[ABSTRACT]BA
2、CKGROUND&OBJECTIVE:Garlicandtheorganosulfercompoundfromgarlichaveantitumoreffects,butthemechanismsstillremainunclear.Thisstudywastoinvestigatethechangesandsignificanceofcaspase-3activityindiallyltrisulfide(DATS)-inducedapoptosisofhumangastriccancercelllineMGC803.METHODS:EffectsofDATSontheapoptosi
3、sofMGC803cellsandthechangeofactivatedcaspase-3wereobservedunderafluorescentandanelectronmicroscopy,anddetectedby南华大学肿瘤研究所,flowcytometryandWesternblot.RESULTS:AfterincubationwithDATS,湖南衡阳421001MGC803cellsshowedtypicalapoptoticmorphologicchanges,andtheapoptosisrateincreasedsignificantly.DATSactivat
4、edcaspase-3inatime-CancerResearchInstitute,dependentmanner:thepositiveratesofactivatedcaspase-3were1.9%,NanhuaUniversity,3.0%,7.3%,14.4%,and27.6%respectivelyinMGC-803cellstreatedwithHengyang,Hunan,421001,12mg/LDATSfor0,4,8,12and24h.Whentreatedwith12mg/LDATSP.R.Chinafor24h,theapoptosisratewassigni
5、ficantlylowerinthecellswithpretreatmentofAc-DEVD-CHO(acaspase-3inhibitor)thaninthecells通讯作者:苏琦withoutpretreatment(5.1%vs.23.0%,P<0.01),indicatingthatAc-DEVD-Correspondenceto:SUQiCHOefficientlyattenuatedDATS-inducedapoptosisofMGC803cells.Tel:86-734-8281547Moreover,pro-caspase3washydrolyzedandactiv
6、atedinDATS-treatedFax:86-734-8281547MGC803cells.CONCLUSION:DATSinducesapoptosisinMGC803cellswhichmaybethroughtheactivationofcaspase-3pathway.E-mail:suqi1@hotmail.comKEYWORDS:Diallyltrisulfide/pharmocology;Gastricneoplasm;MGC803cells;Apoptosis;Caspase-3基金项目:湖南省科研计划重大专项(No.04SK1004);湖南省自然【摘要】背景与目的:
7、大蒜及其烯丙基硫化物具有抗肿瘤作用,但其具体的作科学基金(No.03JJY5002);湖南用机制尚不十分清楚。本研究探讨二烯丙基三硫(diallyltrisulfide,DATS)诱导人省教育厅科研基金重点项目(No.胃癌MGC803细胞凋亡及凋亡过程中Caspase-3的变化及其意义。方法:采用荧光04A047)显微镜和电子显微镜观察以及流式细胞术和Westernblot检测等方法,观察DATS诱导MGC803细胞凋亡的作用及对活
