视黄酸在早期形觉剥夺性近视豚鼠后巩膜中的变化-眼 科研 究20 年

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1、眼科研究2009年12月第27卷第12期1115··实验研究·视黄酸在早期形觉剥夺性近视豚鼠后巩膜中的变化赵小云黄悦赵少贞ChangesofretinoicacidlevelinguineapigscleraofearlyformdeprivationmyopiaZhaoXiaoyun，HuangYue，ZhaoShaozhen．TianjinMedicalUniversityEyeCenter，Tianjin300070，ChinaAbstract0bjectiveResearchesshowedthatretinoicacidmaybeasignalmolecular

2、intheregulationofocularaxiallength．Scleraisatargettissueofthepathogenesisofformdeprivationmyopia．Recently．guineapigsreusedtothestudyofthepathogenesisofformdeprivationmyopia．However，seldomstudiesarefoundinthechangeofretinoicacidlevelinthescleraofguineapigs．Thisstudywasdesignedtoinvestigate

3、theroleofretinoicacidofearlyformdeprivationmyopiainguineapigssclera．MethodsMonocularformdeprivationmodelswereestablishedin243-week—oldguineapigsbyoccludingthelefteyeswithfacemaskandrandomlydividedintoformdeprivation15daysgroupand24daysgroup．Other6normalguineapigswereusedascontrolgroup．The

4、felloweyeswereusedasinternalcontro1．Refractionwasmeasuredbeforeandafterexperimentbyusingretinoscopy．Eyeballswereextractedimmediatelyatthecorrespondingtimepointsandocularaxiallengthwasmeasuredwithoutsidemicrometer．Thelevelofretinoicacidinscleralsampleswasdetectedbyhighperformanceliquidchro

5、matography．TheuseofanimalfollowedtheRegulationsfortheAdministrationofAffairsConcerningExperimentalAnimalsbytheStateScienceandTechnologyCommission．ResultsRefractivediptorreducedby一3．14±1．50Dinexperimentaleyesafterformdeprivation．showingstatisticallydiferencesamongbeforeandvarioustimepoints

6、afterformdeprivation(F=23．053，P=0．000)，andnosignificantdiferencewasseeninreduceofrefractivediptorinfelloweyesafterformdeprivation(一1．24±1．99D)(F=1．506，P=0．240)．Theaxiallengthwasconsiderablyincreasedinformdeprivationeyesafterexperiment(F=14．551，P=0．000)butnoevidentchangeinfelloweys(F=3．612

7、，P=0．060)．Thediopterandocularaxiallengthwassignificantlydifferentbetweenformdeprivationeyesandfelloweyes(P>0．05)．Theexpressionofretinoicacidinscleraofformdeprivationeyeswashigherthanfelloweyesinbothformdeprivation15daysand24days(P<0．01)．Therewasnosignificantdiferenc