重组人血小板源性生长因子增加细胞外信号调节激酶磷酸化促进糖尿病大鼠全层皮肤缺损创面愈合

《重组人血小板源性生长因子增加细胞外信号调节激酶磷酸化促进糖尿病大鼠全层皮肤缺损创面愈合》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。

1、中国修复重建外科杂志2006年第20卷第11期·1093·RecombinantHumanPlatelet-derivedGrowthFactorEnhancesRepairofCutaneousFull-thicknessExcisionbyIncreasingthePhosphorylationofExtracellularSignal-regulatedKinaseinDiabeticRat11222CHENGBiaoLIUHongweiFUXiaobingSHENGZhiyongSUNTongzhu【Abstract】ObjectiveToinvestigatethep

2、ossiblesignalingmechanismsbywhichrecombinanthumanplatelet2derivedgrowthfactor(rhPDGF)acceleratedhealingofcutaneouswoundindiabeticrats.MethodsFourfull2thicknessskinwoundswereincisedinthebackof26maleWistardiabeticrats.Thewoundedratsweredividedinto3groups(7or8ratseachgroup).Onegroupwithouttreat

3、mentwasusedasacontrol,andtheother2groupsweretreatedwith2rhPDGFatadoseof710Lgöcmwoundorvehicle(DMSOö019%NaCl,volövol1∶1)from1to14days.Thewoundhealingwasevaluatedbythemeasurementsofthewoundvolumeandarea.Immunofluorescentandimmunohistochemicalstainingwereusedtoexaminethephosphorylationofextrace

4、llularsignal2regulatedkinase1ö2(ERK1ö2)andtheexpressionofproliferativecellnuclearantigen(PCNA),respectively.ResultsGranulationtissueappearedinthebedofwoundafterinjury.ThenumberofbloodcapillarybudsandfibroblastswasgreaterintherhPDGF2treatedgroupthanthatintheother2groups.Alotofinflammatorycell

5、sinfiltrationandcollagendepositionwereobservedinthewound.Thewound2volumeintherhPDGF2treatedgroupwassmallerthanthatincontrolgroup(P<0.05).ThereepithelializationrateinrhPDGF2treatedgroupwashigherthanthatintheother2groupsat7daysafterinjury(P<0105).TheexpressionofPCNAinreparativecellswashigherin

6、rhPDGF2treatedgroupthanincontrolgrouporvehicle2treatedgroupat3,7daysafterinjury(P<0105).ThephosphorylationofERK1ö2wasstrongerinrhPDGF2treatedgroupthanthatincontrolgrouporvehiclegroupat7and14daysafterinjury(P<0105).ConclusionTheseresultssuggestthatrhPDGFaccelerateswoundhealingandimprovesheali

7、ngqualitybyincreasingthephosphorylationofERK1ö2.【Keywords】DiabeticPlatelet2derivedgrowthfactorWoundhealingExtracellularsignal2regulatedkinaseFoundationitems:NationalBasicScienceResearchandDevelopmentGrants(2005CB522603);NationalNaturalScienceFounda