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1、作物学报ACTAAGRONOMICASINICA2010,36(5):863−870http://www.chinacrops.org/zwxb/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2010.00863水分胁迫下外源ABA提高甘蔗抗旱性的作用机制1,2212,*李长宁ManojKumarSRIVASTAVA农倩李杨瑞12广西大学农学院,广西南宁530004;广西作物遗传改良生物技术
2、重点开放实验室,广西南宁530007摘要:以抗旱甘蔗品种ROC22为材料,调查水分胁迫及胁迫加喷施脱落酸(ABA)对甘蔗内源ABA合成及相关生理生化作用的影响。结果表明,干旱及干旱加外施ABA的条件下,甘蔗内源ABA合成水平上升,但干旱加ABA处理增幅更显著,甘蔗叶内的脯氨酸(Pro)、H2O2、丙二醛(MDA)含量增加,而ABA处理能缓解MDA的积累,使其含量处于低水平,ABA处理能防止叶绿素降解并对干旱引起的最大光能转化效率(Fv/Fm)、PSII实际量子效率(ΦPSII)下降有明显的缓解作用。
3、干旱条件下,H2O2的积累伴随着抗氧化作用的酶CAT、GPX、GR和APX的活性提高,而ABA处理能进一步提高这些相关酶的活性而逐渐降低H2O2的含量,表明干旱条件下,外施ABA能增强甘蔗的抗氧化防护系统,提高抗旱性。关键词:ABA;甘蔗;抗旱性;H2O2;抗氧化酶;叶绿素荧光MechanismofTolerancetoDroughtinSugarcanePlantEnhancedbyFoliageDressingofAbscisicAcidunderWaterStress1,2212,*LICha
4、ng-Ning,ManojKumarSRIVASTAVA,NONGQian,andLIYang-Rui12CollegeofAgriculture,GuangxiUniversity,Nanning530004,China;GuangxiCropGeneticImprovementandBiotechnologyLaboratory,Nanning530007,ChinaAbstract:Droughtisamajorlimitingfactorforsugarcanegrowth.Abscisic
5、acid(ABA)regulatesmuchimportantplantphysio-logicalandbiochemicalprocesses,andinducestolerancetodifferentstressesincludingdrought.UnderstandingthemechanismoftolerancetodroughtinsugarcaneplantwithfoliagedressingofABAunderwaterstresswouldfacilitatebreedin
6、gandfieldman-agementforimprovingdroughtresistance.Thus,anexperimentwassetuptoinvestigatetheinterrelationshipbetweendroughtinducedABAbiosynthesisandantioxidativedefensesystem,andtoconferthefartherroleoffoliarapplicationofABAinimpart-–1ingdroughttoleranc
7、etothesugarcaneplant.Thetreatmentswere:T1(drought),T2(drought+foliarapplicationof15µmolLABA)andC(control,normalirrigation)usingadroughttolerantcultivarROC22.Theresultsshowedthatdroughttreatment(T1)enhancedtheABAconcentrationinleafthatwassignificantlyhi
8、gherincombinedtreatment(T2),suggestingtheABAbiosynthe-siswastriggeredinleafbytheABAapplication.BothT1andT2resultedinanincreaseinproline,H2O2andMDAcontentswhiletheexogenousABAalleviatedtheincreaseinMDAcontent.ABAapplicationdecreasedthede
