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1、ARTICLEINPRESSOpticsandLasersinEngineering44(2006)858–869TherapiddeterminationoffatandproteincontentinfreshrawmilkusingthelaserlightscatteringtechnologyQiXin,HouZhiLing,TianJianLong,YuZhuDepartmentofPhysicsandElectronics,BeijingUniversityofChemicalTechnology,Beijing100029,Chin
2、aReceived22July2003;accepted8February2005Availableonline9February2006AbstractTheaimwastodevelopasimpleandrapidmethodfordeterminationoffatandproteincontentinmilk.Basedonthelaserlightscatteringtheory,theratioofthescatteredlight(at9070.051scatteringangles)intensitytothetransmitte
3、dlightintensity,whichiscalledscattered–transmitted-ratiomethod,isadoptedastheopticalparameterrepresentingthemilkfatcontentandtheproteincontent.Inthisway,theinfluenceofthefluctuationofthepowerofthelightsourceiseliminatedandtheaccuracyofdeterminationisimprovedaccordingly.Thesystem
4、weuseisreal-timeandcansatisfythechallengingrequirementsofdairyfarming.Resultsofthisstudyindicatethefeasibilityofusingthistechnologyforfreshmilkfatandproteinanalysis.Thefatcontentsandproteincontentsof50milksamplesdeterminedbythismethodwereconsistentwiththevaluesobtainedbytheref
5、erencemethodsbasedonRose-GottliebmethodandKjeldahldeterminationofNmethod.Inthispaper,theoperatingprincipleoftheinstrumentisintroducedandtheinfluenceoftheenvironmentalconditions,suchasthehomogenizationpressureandhomogenizationtemperature,etc.ontheresultofthetestisanalyzed.Throug
6、hdataanalysis,theconcreteschemesfortestingthefatusingthecurvefittingandtestingtheproteinusingthesurfacefittingtechniquearedetermined.Finally,thedifferencefromthereferencevaluesofthetestisdiscussed.r2005PublishedbyElsevierLtd.Keywords:Milkfat;Milkprotein;Laserlightscattering;Rapi
7、dmeasurement0143-8166/$-seefrontmatterr2005PublishedbyElsevierLtd.doi:10.1016/j.optlaseng.2005.02.007ARTICLEINPRESSQ.Xinetal./OpticsandLasersinEngineering44(2006)858–8698591.IntroductionRapidanddirectdeterminationoffatandproteincontentsinmilkhavelongbeenawishforbreedingorganiz
8、ation,thedairyindustryaswellasforcontrollaboratories.Currentl
