几种季铵盐阳离子表面活性剂对核盘菌的抗菌活性

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1、万方数据农药学学报2008，10(1)：99-104ChineseJournalofPesticideScience·研究论文·几种季铵盐阳离子表面活性剂对核盘茵的抗茵活性于婷，尚玉珂，石怀兴，慕卫，刘峰‘(山东农业大学植物保护学院，山东泰安271018)摘要：采用茵丝生长速率法测定了5种阳离子表面活性剂对从染病黄瓜植株上分离的核盘茵Sclerotiniasclerotiorum茵丝生长的毒力，及其对茵丝形态、生物量、茵核形成及萌发和茵体细胞膜透性的影响。结果表明，十二烷基苄基二甲基氯化铵抑制菌丝生长的毒力

2、较高，EC∞值为3．92¨g／mL，与杀菌剂乙霉威毒力相当；经阳离子表面活性剂处理后，茵丝粗壮且呈念珠状；随药剂浓度的增大，茵丝生长受抑制的程度增加，茵丝生物量降低率可达90％以上；在阳离子表面活性剂的作用下，茵核的数量与空白对照相比有所减少，但质量略有增加，而腐霉利处理茵核数量无明显变化，但质量明显减少；5种阳离子表面活性剂在10000斗g／mL浓度下均不能完全抑制茵核萌发，而对照药剂腐霉利和嘧霉胺在200斗g／mL即可完全抑制茵核萌发。关键词：阳离子表面活性剂；核盘茵；茵丝生长；毒力；茵核萌发；细胞膜透

3、性中图分类号：$482．2；TQ423．121文献标志码：A．文章编号：1008-7303(2008)Ol-0099-06AntifungalActivityofCationicSurfactantsQuarternaryAmmoniumSaltonSclerotiniasclerotiorumYUTing，SHANGYu—ke，SHIHuai—xing，MUWei，L1UFeng’(CollegeofPlantProtection，ShandongAgriculturalUniversity，Tai’an

4、271018，ShandongProvince，China)Abstract：Theinhibitionactivityonmyceliumgrowthof5cationicsurfactantsand3fungicidesagainst＆如rotiniasclerotiorum(Lib．)deBaryweredeterminedbymyceliumgrowthratemethodinlab．Andtheireffectsonthepathogen’Smyceliumshape，sclerotiumform

5、ation，sclerotiumgermination，dryweightofmyceliumandmembraneosmoticpotentialwerealSOstudied．乃eresultsshowedthatdodecyldimethylbenzylammoniumchloridehadthestrongestinhibitoryeffectonmyceliagrowth，itsEC50valuewas3．92ILg／mL．whichwassimilarwi山fungicidediethofenc

6、arb．Aftertreatedwithcationicsurfactants，themyceliabecamestoutandhadmoniliformappearanoe．Astheconcentrationofthecationicsurfactantsincreased，theinhibitoryeffectonthegrowthandthedryweightofmyceliumbecamestronger．andthemycefium’Sdryweightreducedmorethan90％．An

7、dthesclerotiumnumbersdecreasedcomparedwiththatofthecontrol，howevertheweightincreasedslightly．Incontrast，aftertreatmentwi血procymidone．thesclerotiumnumbershadnovisiblechangebuttheweightdecreasedgreatly．The5cationicsuffactantsevenattheconcentrationof10000斗g／m

8、Lcouldnotrestrainthesclerotiumgerminationcompletely，butthefungicidesofprocymidoneandpyrimethamlcouldrestrainitattheconcentrationof200LL∥IIl】．一．Keywords：cationicsurfactants；Sclerofiniasclerotiorum(Lib)deBary；m