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1、中国环境科学2010,30(2)：209~215ChinaEnvironmentalScience不同DO下MBR内微生物群落结构与运行效果关系*高大文,李昕芯,安瑞,付源,任南琪(哈尔滨工业大学城市水资源与水环境国家重点实验室,黑龙江哈尔滨150090)摘要：应用A/O-MBR处理实际生活污水,考察了不同溶解氧(DO)条件下,微生物群落结构与其处理效果的对应关系.结果表明,DO浓度在+0.2~4.0mg/L对COD去除效果无明显影响,COD平均去除率均在90%以上.DO浓度变化对NH4-N去除影响较大,DO浓度下降到0.2mg/L+时,NH4-N平均去除率由99%下降到65%.通
2、过PCR-DGGE分析,较高DO条件下(4.0,2.0mg/L)的总细菌微生物群落多样性高于较低DO条件(0.5,0.2mg/L),但其群落结构变化与与反应器的处理效果对应关系不明显;氨氧化菌的群落结构变化较明显,且在不同的DO条件下起+主要作用的氨氧化菌的菌属不同,其群落变化与反应器的NH4-N去除效果相对应,DO为2.0,0.5mg/L时氨氧化菌群落结构比较相似,此时反应系统的脱氮效果也比较好.关键词：膜生物反应器；溶解氧；PCR-DGGE；微生物群落结构；氨氧化菌中图分类号：X703.1文献标识码：A文章编号：1000-6923(2010)02-0209-07Relation
3、shipsbetweenmicrobialcommunitystructureandtheperformanceofMBRunderdifferentdissolved*oxygen.GAODa-wen,LIXin-xin,ANRui,FUYuan,RENNan-qi(1.StateKeyLaboratoryofUrbanWaterResourceandEnvironment,HarbinInstituteofTechnology,Harbin150090,China).ChinaEnvironmentalScience,2010,30(2)：209~215Abstract：Mic
4、robialcommunitystructureandnutrientremovalperformanceunderdifferentdissolvedoxygenwereinvestigatedusingAnoxic-oxicsubmergedmembranebioreactor(A/O-MBR)formunicipalwastewatertreatment.ResultsshowedthattheCODremovalwasnotinfluencedmarkedlybythevariationsinDObetween0.2mg/Land4.0mg/L,andtheCODremov
5、alwasmorethan90%duringthewholeexperiment.However,theammonianitrogenremovalwasmoresensitivethanCOD,andtheammonianitrogenremovaldroppedfrom99%to65%whenDOdecreasedto0.2mg/L.ThePCR-DGGEanalysisshowedthatthediversityoftotalbacterialcommunitywasabundantwhenA/O-MBRwasrunathighDO(4.0mg/Land2.0mg/L),an
6、dtherelationshipsbetweenthetotalbacterialcommunitystructureandtheperformanceofMBRwasnotveryobvious.Onthecontrary,theammoniaoxidizingbacterialcommunitycompositionhadamoreobviousshiftwiththevariationofDOconcentration,andthespeciesofammoniaoxidizingbacteriawhichplaymajorroleweredifferentunderdiff
7、erentDOconditions,whichwerecorrespondedtotheremovalefficiency.TheammoniaoxidizingbacterialcommunitycompositionweresimilarundertheDOconcentrationof2.0mg/Land0.5mg/L,andthenitrogenrenmovalwasenhanced.Keywords：membranebioreactor(MBR)；disso
