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1、SPECIALSECTION:PLANTMOLECULARBIOLOGYCurrentinitiativesinproteomicsresearch:TheplantperspectiveHimanshuDubeyandAnilGrover*DepartmentofPlantMolecularBiology,UniversityofDelhiSouthCampus,BenitoJuarezRoad,DhaulaKuan,NewDelhi110021,Indiascience.ItparticularlyemphasizestheapplicationofTherecent
2、upsurgeinstructuralgenomicsisleadingproteomicsinisolationofimportantnovelplantgenes.totheaccumulationofahugewealthofliteratureonnucleotidesequences.AfterthenucleotidesequenceofagivenstretchofDNAisobtained(bymanualorProteomicsresearchroboticmethods),thenextstepistousesomesoft-wareprogramto
3、distinguishthepossibleopenread-Genomicsisthemostrecenthappeningscienceiningframesinthethickofsequences.However,the4,5contemporarybiology.Completegenomesofmoreacidtest,whetherthenewsequencecorrespondstoanyfunctionalityintermsoftranscriptionandtrans-than30organisms(e.g.Escherichiacoli,Bacil
4、lussub-lationistoidentifytheproteinwhichitencodes.tilis,Synechocystissp.,Mycobacteriumtuberculosis,Functionalgenomicsandproteomicsarethebuzz-Treponemapallidum,Borreliaburgdorferi,Deinococ-wordsinmodern-daygenomics.Thescienceofpro-cusradiodurans,Aquifexaeolicus,Saccharomycescer-teomicsisap
5、ossibleapproachtorelatetheskeletalevisiaeandCaenorhabiditiselegans)havealreadybeennucleotidesequenceinformationtofunctionalat-sequencedandthegenomesofanother100orsoorgan-tributesofthecell.Theidentificationandisolationisms,includingthatofhuman,arebeingsequencedataofnovelgeneswithpotentialb
6、iotechnologicalappli-rapidpace.Amongplants,thegenomeofArabidopsiscationswarrantthatgenomicsandproteomicsmustthalianahasalreadybeencompletelysequencedandthegohandinhand.ThreemajorstepsinproteomegenomeofOryzasativaisatargetforcompletenucleo-analysisaretheseparationofcomplexproteinmix-tidese
7、quencingwithinthenext34years.Apartfromturesbytwo-dimensionalproteingelelectrophoresis(2D),characterizationoftheseparatedproteinsbythese,thereisagreatdealofprogressingenomicsofmassspectrometer(MS)anddatabasesearching.maize,sorghum,sugarcane,barley,cotton,tomato,soy-T