资源描述：
《代谢工程 英文原版PDF Chapter 12 - Analysis of Structure of Meta》由会员上传分享，免费在线阅读，更多相关内容在行业资料-天天文库。
1、CHAPTER12AnalysisofStructureofMetabolicNetworksInthepreviouschapter,wereviewedthebasicconceptsofMetabolicControlAnalysisandmethodsforthedeterminationofitskeyparameters.ItwasnotedtherethattheframeworkofMCAprovidesthemeansofquantify-ingthedegreeofcontrolexertedbyeachindividualreactionstepina
2、metabolicnetworkuponeachnetworkfluxandmetabolite,intheformofcontrolcoefficients.Ineffect,controlcoefficientsprovideacharacterizationoftheeffectsresultingfromtheperturbationofaparticularreaction,sothattheeffectsofotherperturbationsinthatsamestepcanbepredicted.ThemethodspresentedinChapter11p
3、ermitthedeterminationofcontrolcoefficientsandotherMCAparametersfromfluxestimates,evenafterlargeperturbationsinthekineticsofthenetwork.Chapters8-10providedacomprehensiveapproachtotheestimationoffluxesrequiredforthesecalculations,baseduponmetabolitebalances,reactionstoichiometries,andisotopi
4、clabels.535536MetabolicEngineeringInthischapterweturntothechallengingtaskofanalyzingfluxcontrolinmorecomplexnetworksthanthesimpleunbranchedorbranchedpathwaysdiscussedinChapter11.Wedefineacomplexmetabolicnetworkasasystemconsistingofmanyenzymaticreactionsproducinganddepletingmetabolitesandin
5、teractingwithoneanotherthroughcommonprecursors,products,oreffectors.Suchnetworksprocessexternalsubstratesthroughaseriesofreactionswhichultimatelyproduceoneormoredesirableproducts,oftenincompetitionwithunwantedbyproducts.Anexample,whichwillbeusedasacasestudythroughoutthisandthefollowingchap
6、ter,isthearomaticaminoacidbiosyntheticpathway(Fig.12.1).Withtheuseofamodelforthenetworkreactionkinetics(StephanopoulosandSimpson,1997),wewillillustratethemethodsallowingexperimentalelucidationofthecontrolstructureofthisnetwork,oranysimilarlycomplexsystem.Theobjectivesofthisandthefollowingc
7、hapteraretoprovidegeneralandsystematicmethodsfor 9definingtheintrinsicstructureofanetwork,intermsofidentifiablebranchpoints,baseduponthesystem'sstoichiometry; 9analyzingthekineticbehaviorofcomplexmetabolicnetworksintermsofMCAparameters; 9identifyingcriticalrea
