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1、CompleteChemicalSynthesis,Assembly,andCloningofaMycoplasmagenitaliumGenomeDanielG.Gibson,etal.Science319,1215(2008);DOI:10.1126/science.1151721Thefollowingresourcesrelatedtothisarticleareavailableonlineatwww.sciencemag.org(thisinformationiscurrentasofApril13,2008):Updatedinformationa
2、ndservices,includinghigh-resolutionfigures,canbefoundintheonlineversionofthisarticleat:http://www.sciencemag.org/cgi/content/full/319/5867/1215SupportingOnlineMaterialcanbefoundat:http://www.sciencemag.org/cgi/content/full/1151721/DC1AlistofselectedadditionalarticlesontheScienceWebsi
3、tesrelatedtothisarticlecanbefoundat:http://www.sciencemag.org/cgi/content/full/319/5867/1215#related-contentThisarticlecites29articles,16ofwhichcanbeaccessedforfree:http://www.sciencemag.org/cgi/content/full/319/5867/1215#otherarticlesonApril13,2008Thisarticleappearsinthefollowingsub
4、jectcollections:Geneticshttp://www.sciencemag.org/cgi/collection/geneticsInformationaboutobtainingreprintsofthisarticleoraboutobtainingpermissiontoreproducethisarticleinwholeorinpartcanbefoundat:http://www.sciencemag.org/about/permissions.dtlwww.sciencemag.orgDownloadedfromScience(pr
5、intISSN0036-8075;onlineISSN1095-9203)ispublishedweekly,exceptthelastweekinDecember,bytheAmericanAssociationfortheAdvancementofScience,1200NewYorkAvenueNW,Washington,DC20005.Copyright2008bytheAmericanAssociationfortheAdvancementofScience;allrightsreserved.ThetitleScienceisaregisteredt
6、rademarkofAAAS.RESEARCHARTICLEgenome,weneededtoestablishconvenientandreliablemethodsfortheassemblyandcloningofmuchlargersyntheticDNAmolecules.CompleteChemicalSynthesis,Assembly,Strategyforsynthesisandassembly.Thena-tive580,076-bpM.genitaliumgenomesequenceandCloningofaMycoplasma(Mycop
7、lasmagenitaliumG37ATCC33530genomicsequence;accessionno.L43967)(3)genitaliumGenomewaspartitionedinto101cassettesofapproxi-mately5to7kbinlength(Fig.1)thatwereindividuallysynthesized,verifiedbysequencing,DanielG.Gibson,GwyneddA.Benders,CynthiaAndrews-Pfannkoch,EvgeniyaA.Denisova,andthen
8、joinedtogeth
