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1、复旦学报(医学版)FudanUnivJMedSci2011Jul.,38(4)303血小板衍化生长因子及其受体在糖尿病下肢动脉硬化闭塞症中的表达及意义122李旭邵明哲范隆华(1复旦大学附属中山医院青浦分院普外科上海201700;2复旦大学附属中山医院血管外科上海200032)摘要!目的探讨2型糖尿病伴下肢动脉硬化闭塞症(arteriosclerosisobliterans,ASO)动脉硬化斑块中血小板衍化生长因子(plateletderivedgrowthfactor,PDGF)及其受体(PDGFR)的表达及意义。方法2007年1月至2009年1
2、2月复旦大学附属中山医院血管外科行介入治疗或手术治疗的ASO患者共22例,其中合并2型糖尿病者13例,无糖尿病者9例。共获取动脉硬化标本27个。对行手术治疗的ASO患者留取动脉内膜斑块,分别进行苏木精伊红(HE)染色及免疫组化染色,通过光学显微镜了解PDGFR和PDGFR在动脉硬化标本中的表达。RTPCR法检测PDGFA、B链mRNA。结果无糖尿病的ASO患者下肢动脉硬化斑块经HE染色可见少量脂质浸润,免疫组化染色见PDGFR、少量表达;2型糖尿病伴下肢ASO患者下肢动脉硬化斑块,HE染色可见大量脂质浸润,免疫组化染色可见PDGFR、均大量表达。2
3、型糖尿病伴下肢ASO患者髂、股动脉段动脉硬化标本中PDGFB链mRNA的表达远远高于其在无糖尿病者中的表达,PDGFB链在腘动脉段的表达较髂、股动脉段明显增加。结论2型糖尿病伴ASO患者下肢动脉硬化标本中存在PDGFR、高表达,腘动脉标本中PDGFB链数量增多,提示该因子在此类患者中的病情进展中发挥重要作用。关键词!动脉硬化闭塞症;血小板衍化生长因子;糖尿病;动脉硬化斑块中图分类号!R587.1文献标志码!Adoi:10.3969/j.issn.16728467.2011.04.005Expressionandsignificanceofp
4、lateletderivedgrowthfactoranditsreceptorsinarteriosclerosisplaqueontype2diabetesmellituscombinedwitharteriosclerosisobliteransoflowerextremity122LIXu,SHAOMingzhe,FANLonghua1(DepartmentofGeneralSurgery,QingpuBranchofZhongshanHospital,FudanUniversity,2Shanghai201700,China;DepartmentofVas
5、cularSurgery,ZhongshanHospital,FudanUniversity,Shanghai200032,China)Abstract!ObjectiveToexploretheexpressionofplateletderivedgrowthfactor(PDGF)anditsreceptorsinarteriosclerosisplaqueontype2diabetesmellitus(T2DM)combinedwitharteriosclerosisobliterans(ASO)oflowerextremity.MethodsFromJa
6、n.2007toSept.2009,therewere22patientswithASOreceivedinterventionaltherapyorsurgicaloperationinZhongshanHospital,FudanUniversity,amongwhich13caseswerecombinedwithT2DM.Weobtained27samplesofarterioscleroticplaquesfromthesepatients.OnepartofsamplesweredyedwithHEandimmunohistochemicalstainingto
7、researchonPDGFreceptor(PDGFR),theothersweredetectedforAandBchainmRNAexpressionsofPDGFbyRTPCR.ResultsAlittlelipidinvasionwasfoundinthesamplesoffemoralarteriosclerosisplaquesfromASOpatientswithoutT2DMbyHEstaining,andafewPDGFRandwasexpressedinfemoralarterio
