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1、中国水稻科学(ChinJRiceSci),2013,27(2):145-152http://www.ricesci.cnDOI:10.3969/j.issn.1001-7216.2013.02.006145水稻单片段代换系对两种不育细胞质恢复性的遗传分析蔡健1,2代资举2朱海涛2曾瑞珍2张泽民2王敉敉1马同富1,*张桂权2,*(1阜阳师范学院生命科学学院,安徽阜阳236041;2华南农业大学植物分子育种重点实验室,广东广州510642;*通讯联系人,E-mail:gqzhang@scau.edu.cn;matongfu6328@126.com)GeneticAnal
2、ysisofRestorationofChromosomeSingleSegmentSubstitutionLinetotwoDifferentCMSLinesinRiceCAIJian1,2,DAIZi-ju2,ZHUHai-tao2,ZENGRui-zhen2,ZHANGZe-min2,WANGMi-mi1,MATong-fu1,*,ZHANGGui-quan2,*(1SchoolofLifeSciences,FuyangTeachersCollege,Fuyang236041,China;2StateKeyLaboratoryforConservationan
3、dUtilizationofSubtropicalAgro-bioresources,SouthChinaAgriculturalUniversity,Guangzhou510642,China;*Correspondingauthor,E-mail:gqzhang@scau.edu.cn;matongfu6328@126.com)CAIJian,DAIZiju,ZHUHaitao,etal.GeneticanalysisofrestorationofchromosomesinglesegmentsubstitutionlinetotwodifferentCMSli
4、nesinrice.ChinJRiceSci,2013,27(2):145-152.Abstract:Aricechromosomesinglesegmentsubstitutionline(SSSL)S42,whichisastrongrestorerlineforwildabortivecytoplasmicmalesterility(WA-CMS)andY-CMS,wasidentifiedattheGuangdongProvincialKeyLaboratoryofPlantMolecularBreeding,SouthChinaAgriculturalUn
5、iversity.Thegeneticmodeoffertilityrestorer(Rf)genesandthegeneticrelationshipbetweenWA-CMSandY-CMSsystemswerecharacterizedthroughtheproductionofbackcrosspopulations.TheSSSLS42wasusedtopollinateWA-CMSlineofZhenshan97AandY-CMSlineofY-HuanongAandgeneratedtwoBC3F2populationsbyusingmarker-as
6、sistedselectionandtraditionalbackcrossing.Resultswereshownasthefollowing:1)InthegeneticbackgroundofS42,WA-CMSwasrestoredmoreeasilythanY-CMS,andtheeffectofRf4appearedtobeslightlylargerthanthatofRf3forthetwoCMSsystems;2)TwopairsofdominantgenesRf3andRf4governedpollenfertilityrestoration,a
7、ndmodifyingorminorgenesalsoinvolvedintheinheritanceofrestorerabilityinS42,whichindicatesthatthegeneticmodeofRfgeneswasaqualitative-quantitativecharacterforWA-CMSandY-CMSsystems;3)When205SSRmarkerswereusedtoanalyzethegeneticbackgroundandaveragelengthsubstitutedchromosomesegments(SCS)o
