资源描述:
《复方黄芪制剂对鸡新城疫灭活疫苗免疫效果的影响》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、2015年第36卷第1期增刊复方黄芪制剂对鸡新城疫灭活疫苗免疫效果的影响111112■刘明生甘辉群贺生中傅宏庆谭菊徐勇(1.江苏农牧科技职业学院,江苏泰州225300;2.江苏苏鹏禽业发展有限公司,江苏海安226661)摘要:为探讨复方黄芪制剂对鸡新城疫灭活疫苗免疫效果的影响,将300只7日龄海兰褐雏鸡随机分成Ⅰ组(低剂量添加组)、Ⅱ组(中剂量添加组)、Ⅲ组(高剂量添加组)、Ⅳ组(阴性对照组)和Ⅴ组(阳性对照组)。7日龄时,除阴性对照组不免疫外,其余各组鸡均颈背部皮下注射新城疫灭活疫苗,0.25ml/羽,同时Ⅰ、Ⅱ和Ⅲ组分别按10g/kg、20g/kg和40g/kg体重
2、,灌服复方黄芪制剂,每天1次,连续5d。然后在免疫后第7、14、21、28d和35d时,从各组中随机选取8只健康鸡无菌采集心血1.0ml,分离血清,用于鸡新城疫HI抗体、SOD和IFN-γ的测定。结果表明:新城疫HI抗体效价,血清中SOD和IFN-γ的活性或浓度,复方黄芪中剂量均高于其余各组,且与低剂量添加组、阳性对照组相比,呈显著(P<0.05)或极显著差异(P<0.01)。说明复方黄芪按20g/kg体重的剂量给雏鸡口服,能明显提高雏鸡的新城疫HI抗体水平,同时也能提高雏鸡SOD和IFN-γ的水平关键词:复方黄芪;鸡新城疫灭活疫苗;ND-HI抗体效价;SOD;IFN-
3、γdoi:10.13302/j.cnki.fi.2015.Z1.016中图分类号:S816.32文献标识码:A文章编号:1001-991X(2015)Z1-0059-04EffectsofcompoundastragaluspreparationsontheimmunityefficacyoftheinactivatedvaccineagainstnewcastlediseaseinchickensLiuMingsheng,GanHuiqun,HeShengzhong,FuHongqing,TanJu,XuYongAbstract:Toinvestigatetheeff
4、ectsofcompoundastragaluspreparationsontheimmunityefficacyofthein⁃activatednewcastlediseasevaccineinchickens,the300sevenday-oldHaiLanchickenswererandomlydi⁃videdintogroupⅠ(addinglow-dosegroup),groupⅡ(addingmoderate-dosegroup),groupⅢ(addinghigh-dosegroup),groupⅣ(negativecontrolgroup)andgro
5、upⅤ(positivecontrolgroup).Whenthechickenswere7daysofage,theyweresubcutaneousinjectedtheinactivatednewcastlediseasevaccineex⁃ceptthenegativecontrolgroup,eachof0.25ml.Meanwhile,thechickensinthegroupⅠ,groupⅡandgroupⅢwereoralgavagedthecompoundastragaluspreparationsas10g/kg,20g/kgand40g/kgbod
6、yweight,oncedailyfor5consecutivedays.Thenatthe7,14,21,28dand35dafterimmunizing,eighthealthychickenswererandomlyselectedfromeachgroupand1.0mlbloodwerecollectedsterilizing,serumwasseparatedforthedetectionoftheND-HIantibody,SODandIFN-γ.Theresultsareasfollows:thenew⁃castlediseaseHIantibodyle
7、vels,serumactivityorconcentrationoftheSODandIFN-γ,thecompoundas⁃tragalusmoderate-dosegroupwashigherthantheothergroups,andcomparedwiththelow-dosegroupandthepositivecontrolgroup,itwasasignificantorverysignificantdifference.Thestudyshowthatthecom⁃poundastragaluspreparationsw