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1、作物学报ACTAAGRONOMICASINICA2016,42(2):243254http://zwxb.chinacrops.org/ISSN0496-3490;CODENTSHPA9E-mail:xbzw@chinajournal.net.cnDOI:10.3724/SP.J.1006.2016.00243地黄连作胁迫响应机制的块根蛋白质组学分析1,21,21,21,21,22吴林坤陈军吴红淼王娟英秦贤金张重义1,2,*林文雄12福建农林大学生命科学学院,福建福州350002;福建省农业生态过程与安全监控重点实验室,福建福州350002摘要:地黄种植过程中存在
2、严重的连作障碍问题,连作导致块根无法正常膨大、产量品质下降、土传病害严重等。本研究以正、重茬地黄块根为试验材料,通过差异蛋白质组学技术分析连作下地黄块根蛋白质表达谱变化,并进一步采用qRT-PCR技术对锁定的差异蛋白质表达量变化进行验证分析。研究结果发现,连作导致与块根重要生理代谢过程和主要成分合成相关的蛋白质都下调表达,与蛋白质折叠相关的伴侣素(chaperonin)在重茬地黄块根中全部下调表达;连作下与胁迫响应、抵御相关的蛋白质(如pathogenesis-relatedprotein10,cytochromeP450,TypeIIIamembraneprote
3、incp-wap13等)均上调表达。qRT-PCR定量分析证实重茬地黄块根中PR-10基因表达量显著高于正茬地黄;PR-10基因在尖孢镰刀菌病原菌侵染下能够明显被诱导表达,表达量随着侵染时间的增加而逐渐升高,验证了差异蛋白质组学分析的结果。可见连作胁迫对地黄块根蛋白质表达谱有显著影响,导致蛋白质表达紊乱,连作植株生理代谢过程异常,碳水化合物和能量代谢缓慢,产生连作障碍效应。关键词:地黄;连作障碍;差异蛋白质组学;病程相关蛋白;胁迫响应ComparativeProteomicsAnalysisofR.glutinosaTuberRootinResponsetoCons
4、ecutiveMonoculture1,21,21,21,21,22WULin-Kun,CHENJun,WUHong-Miao,WANGJuan-Ying,QINXian-Jin,ZHANGZhong-Yi,1,2,*andLINWen-Xiong12CollegeofLifeSciences,FujianAgricultureandForestryUniversity,Fuzhou350002,China;FujianProvincialKeyLaboratoryofAgroecologicalProcessingandSafetyMonitoring/Fujia
5、nAgricultureandForestryUniversity,Fuzhou350002,ChinaAbstract:RehmanniaglutinosaL.iswidelyappliedinChinesemedicine.However,consecutivemonocroppingofthisplantre-sultsinseriousdeclineinbothbiomassandqualityofundergroundtubers,withpoorfieldperformanceandinsufficientresistancetodiseaseandpe
6、st.Inthepresentstudy,thetuberrootsfromthenewlyplanted(NP)andconsecutivelymonocropped(SM)R.glutinosawereusedthroughcomparativeproteomicsanalysistostudytheresponseofR.glutinosatoconsecutivemonocroppingandtheunderlyingmechanismsofreplantingdisease.Comparativeproteomicsanalysisshowedthatth
7、eseproteinsinvolvedinimportantphysiologicalprocessesandbiosyntheseofmaincomponentsintuberrootsweresignificantlydown-regulatedunderconsecutivemonocroppingregime.Itwasalsofoundthatchaperoninsrelatedtoproteinfoldingwasdown-expressedwiththeextendedmonocropping.However,theseproteinsrelate
