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1、1、Westernblotting:WesternblottingusingECLreagentusedby：LaboratoryofP.J.Hansen,Dept.ofAnimalSciences,UniversityofFloridaKamps'sWesternBlottingProtocolusedby：SeftonLab,SalkInstituteDryTransfer干法蛋白转膜usedby：ThePreussLab,TheDivisionofBiologicalSciences,TheUniversityofChicagoWesternBl
2、otofTBPfromTBP-GSTbacteriausedby：StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSFWesternBlot/Anti-P-CREBusedby：StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSFWesternblottingprotocolfor1C2,3B5H10,and4C8Antibodiesusedby：StevenFinkbeiner,DepartmentsofNeurologyand
3、Physiology,UCSFProtocolforanti-HAantibodyWesternBlottingusedby：StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSFProbingandStrippingofWesternBlotusedby：HahnLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstituteWesternBlottingProtocolusedby：MirmiraLaborat
4、oryattheUniversityofVirginiaWesternBlottingandImmunostainingusedby：ChazinLab,CenterforStructuralBiology,VanderbiltUniversityWesternBlotsusedby：LabofDr.MarkBartonFrank,OklahomaMedicalResearchFoundationWesternBlotting-Antibodiesusedby：TheMinionLab,CollegeofVeterinaryMedicineatIowa
5、StateUniversityWesternBlottingusedby：Cepko/TabinLab,HarvardUniversityCarbonateSolutionForWesternBlotusedby：Dr.DEKoshland,CarnegieInstitutionofWashingtonWesternBlotwithBSAusedby：Dr.DEKoshland,CarnegieInstitutionofWashingtonMETHODforWesternBlotsusedby：Dr.DEKoshland,CarnegieInstitu
6、tionofWashingtonWesternBlotsusedby：MichaelKoelle'sLab,DepartmentofMolecularBiophysicsandBiochemistryYaleUniversity2、蛋白分离/提取/浓缩LysisBuffersusedby：StevenFinkbeiner,DepartmentsofNeurologyandPhysiology,UCSFYeastProteinIsolation"Yaffe-Schatz"usedby：AmbergLab,UpstateMedicalUniversityG
7、uanidineHydrochloridePurificationofProteinsFromSDSPAGEusedby：GottschlingLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstituteYeastproteinprepforSDSPAGEandwestern(rapid)usedby：GottschlingLab，TheFredHutchinsonCancerResearchCenterandHowardHughesMedicalInstituteYe
8、astproteinprepforSDSPAGEandwestern(glassbead)us
