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时间:2019-05-18
《青少年特发性脊柱侧凸患者软骨细胞中褪黑素受体的表达量》由会员上传分享,免费在线阅读,更多相关内容在行业资料-天天文库。
1、中国脊柱脊髓杂志2009年第l9卷第3期ChineseJournalof却ineandSpinolCord,2009,Vo1.19,No.3227青少年特发性脊柱侧凸患者软骨细胞中褪黑素受体的表达量孙光权,邱勇,黄爱兵,李海波,邱旭升(南京大学医学院附属鼓楼医院脊柱外科210008南京市)【摘要】目的:观察青少年特发性脊柱侧凸(adolescentidiopathicscoliosis,AIS)患者软骨细胞中褪黑素受体含量的变化,并探讨其与AIS病因学的关系。方法:随机选取2007年1月~2007年1
2、2月在我院手术治疗的22例AIS患者及8例非脊柱侧凸(肿瘤、外伤等)患者(对照组)。AIS组患者年龄10~16岁,平均12.7岁,平均Cobb角60.1o,其中10例在术中取髂软骨,12例取棘突软骨。对照组患者年龄10~15岁,平均12.9岁,其中7例在术中取髂软骨,1例取棘突软骨。对髂软骨和棘突软骨标本进行细胞培养至二代,取二代细胞用Ⅱ型胶原免疫组化染色方法进行软骨细胞表型鉴定,并抽提总RNA,采用逆转录聚合酶链反应(RT—PCR)检测软骨细胞中褪黑素受体两种亚型MTNR1A、MTNR1B的mRNA
3、表达量。结果:两组患者软骨细胞Ⅱ型胶原免疫组化染色均为阳性表达。AIS组软骨细胞中MTNR1A、MTNR1BmRNA的相对表达量分别为0。44_+0.29和0.54+0.31,对照组分别为0.69+0.18和0.83±0.30,组间比较均有显著性差异(P4、图分类号:R682.3,Q735文献标识码:A文章编号:1004—406X(2009)一03—0227一O4Expressionofmelatoninreceptorsinchondrocyteofadolescentidiopathicscoliosispatients/SUNGuangquan,QIUYong,HUANGAibing,etaF/ChineseJournalofSpineandSpinalCord,2009,19(3):227-230【Abstract】Objective:Toinv5、estigatetheexpressionofmelatoninreceptorsinchondrocytederivedfromado—lescentidiopathicscoliosis(AIS)patients,andexploretheroleofmelatonininthepathogenesisofscoliosis.Method:FromJanuary2005toDecember2005,twogroupsweredefinedasAISgroupandcontrolgroup.AISg6、roupconsistedof22patientswithanaverageageof12.7years,rangingfrom10to16years.TheaverageCobbanglewas60.1o.10patientsiliaccartilageand12patientsspinalprocesscartilageweretakenduringspinalsurgery.Controlgroupconsistedof8subjectswithanaverageageof12.9years,r7、angingfrom10to15yearswhounderwentsurgerybecauseofbenigntumors,trauma.7patientsiliaccartilageand1patientsspinalproeesscartilageweretakenduringsurgery.Thechondrocytefromiliaccartilageandspinalprocesscartilagewerecuhivatedandseria1subcuhivatedinvitro.Immun8、ohistochemicalstainofcollagenⅡwereperformedtoconfirmtheP2chondrocytephenotype.ThemRNAexpressionofmelatoninrecepterIA(MTNR1A)andmela—toninreceptor1BfMTNR1B)inchondrocyteweredetectedbyRT—PCRmethod.Result:Thecollagentype11immunohist
4、图分类号:R682.3,Q735文献标识码:A文章编号:1004—406X(2009)一03—0227一O4Expressionofmelatoninreceptorsinchondrocyteofadolescentidiopathicscoliosispatients/SUNGuangquan,QIUYong,HUANGAibing,etaF/ChineseJournalofSpineandSpinalCord,2009,19(3):227-230【Abstract】Objective:Toinv
5、estigatetheexpressionofmelatoninreceptorsinchondrocytederivedfromado—lescentidiopathicscoliosis(AIS)patients,andexploretheroleofmelatonininthepathogenesisofscoliosis.Method:FromJanuary2005toDecember2005,twogroupsweredefinedasAISgroupandcontrolgroup.AISg
6、roupconsistedof22patientswithanaverageageof12.7years,rangingfrom10to16years.TheaverageCobbanglewas60.1o.10patientsiliaccartilageand12patientsspinalprocesscartilageweretakenduringspinalsurgery.Controlgroupconsistedof8subjectswithanaverageageof12.9years,r
7、angingfrom10to15yearswhounderwentsurgerybecauseofbenigntumors,trauma.7patientsiliaccartilageand1patientsspinalproeesscartilageweretakenduringsurgery.Thechondrocytefromiliaccartilageandspinalprocesscartilagewerecuhivatedandseria1subcuhivatedinvitro.Immun
8、ohistochemicalstainofcollagenⅡwereperformedtoconfirmtheP2chondrocytephenotype.ThemRNAexpressionofmelatoninrecepterIA(MTNR1A)andmela—toninreceptor1BfMTNR1B)inchondrocyteweredetectedbyRT—PCRmethod.Result:Thecollagentype11immunohist
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