沙利度胺对类风湿关节炎患者VEGF表达的影响

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1、硕十学位论文中文摘要摘要目的：检测类风湿关节炎(RheumatoidArthritis，RA)患者血清和外周血单核细胞(peripheralbloodmononuclearcells，PBMCs)血管内皮生长因子(VascularEndothelialGrowthFacter,VEGF)表达，观察沙利度胺(Thalidomide，THD)对PBMCsVEGF表达的影响，探讨THD治疗RA的可能机制。方法：抽取RA患者及正常人外周静脉血，ELISA法测定血清VEGF含量，密度梯度离心法获得PBMCs，RT-PCR检测PBMCsVEGFmRNA表达。MTT法检测THD对RA患者PBMCs的增殖抑

2、制率。将培养的RA患者PBMCs随机分成6组，用不同浓度的THD(O}tg／ml、lOItg／ml、50pg／ml、100}tg／ml、200pg／ml、400}tg／m1)干预24h，RT-PCR检测PBMCsVEGFmRNA表达，ELISA法检测PBMCs上清液中VEGF水平。结果：(1)RA患者血清VEGF平均水平较正常对照组明显升高，(P

3、类风湿关节炎的机制可能是与抑制RA患者PBMCs增殖和VEGF表达，减少新生血管的形成有关。关键词：沙利度胺，类风湿关节炎，单核细胞，血管内皮生长因子硕士学位论文英文摘要ABSTRACTObjective：ToinvestigatethepossiblemechanismofthalidomidewhichcantreatRApatients．WedetectedtheexpressionlevelsofVEGFinSerumandperipheralbloodmononuclearcellsandtheeffectsofthalidomideonperipheralbloodmononuc

4、learcellsofVEGFinpatientswithrheumatoidarthritis．Methods：Peripheralvenousbloodsamplesofhealthyadultsandpatientswithrheumatoidarthritiswerecollectedrespectively,serumVEGFvaluesweremeasuredbyELISA，PBMCswereisolatedbymeansofdensitygradientcentrifugation，theexpressionofVEGFmRNAinPBMCsweredetectedbysemi

5、．quantitativeImPCR．thalidomidewereaddedtodetecttheratesofvalue．addedinPBMCcellswhichinhibitedbyMTT．PBMCCellswererandomlydividedintosixgroupsandweregivendifferentconcentrationofthalidomide(Oug／ml，10ug／ml，50ug／mL，lOOug／mL，200ug／mL，400ug／mL)．Aftertheinterventionfor24hours，theexpressionofVEGFmRNAinPBMC

6、sweredetectedbysemi．quantitativeIU∑PCR．thelevelsofVEGFinPBMCssupernatantweredetectedbvELISA．Results：(1)SerumVEGFconcentrationsinpatientswithRAweresignificantlyhigherthanthatinhealthyadults(P<0．05)：(2)TheexpressionofVEGFmRNAinPBMCsofRA．whichwassignificantlyhigherthantheexpressionofVEGFmRNAinnormalco

7、ntrol(P<0．05)：(3)ThalidomidesignificantlyinhibitedtheproliferationofPBMCs(P<0．05)；(4)ThalidomideinhibitedthesecretionofPBMCsVEGFinadose-dependentmanner．(p