递增运动负荷训练对大鼠心房肌蛋白质组影响的研究

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1、湖南师范大学硕士学位论文递增运动负荷训练对大鼠心房肌蛋白质组影响的研究姓名：龚丽申请学位级别：硕士专业：运动人体科学指导教师：史绍蓉20070501AbstractThepurposeofthisstudyistodiSCUSStheinfluencewhichauricularmuscleproteonncsOnincreasingprogressivelyexercise‘load。trammg，sievetargetedproteinofvariabilityexpressedandimportantsignificanceinsport，investigationruleof

2、developmentandefflUenCeofsplortsfatiguemproteome．Applyingtheoryandtechniqueofproteome，tenmaleSDratsweredividedintotwogroupsrandomly：therestgroup(5)andtheexercisegroup(5)．Afterincreasingprogressivelyexercise-load-training7weeks，exhaustingtraining6hours，theywerenarcotizationexecutedatthesalnetime

3、．Extractedwholeproteinofauricularmuscle，theproteinswereseparatedby2-DE，stainedbyCoomassiebrilliantbluedye，analyzedbyMALDI．TOF—TOF．MSinturn．354+40and382士24proteinspotswerefoundinthesportgroupandtestgroup,matclfingratewere68．67％士9．87％and72．67％士8．62％．coefficientcorrelationwere0。7l：illthe104differe

4、ntiallyexpressedspotsaftertraning，66inup-regulation2times，including18inup-regulation5times，noneproteinspotinincrease；38indown-regnlation2times，including23indown-regulation5times，7inobsolescented．Selectedproteinspotsofdifferentialexpressedmore5timesasobjectprotemum,therewremasschromatograptncana

5、lysis，5proteinspotswereassessmented：myosinlightpolypeptide4andaspartateaminotransferasewereup-regulation,Myosin-6Wasdown-regulationandabsence．Experimentalresultindicatedthatauricularmuscleproteinwereobviouslyvariabilityexpressed，anditWaSsufficientexperimentbaseinordertoprofoundlystudyoriginalob

6、jectiveproteinumofsportsfatigue．Theauricularmuscleproteinofvariabilityexpressedarerelatedtosignaltransduction，energymetabolism,substancemetabolismandmyocardialfunction．田lcdisappearedanddowm-regulatedproteinsinexercisegroupareralatedtodecreaseofmusclecontractionandmotorabilityandproceedofsportsf

7、atigue；theup-regulationareralatedtoinjuryofhistiooyticinlong-termmereasmgprogressivelyexercise。load-trmmng。Mostimportant，myosinlightpolypeptide4andaspartateaminotransferasethatsuccessfullysievethroughmethodofproteomewillimpactorga