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ID:36641246
大小:1.71 MB
页数:48页
时间:2019-05-13
《递增运动负荷训练对大鼠心房肌蛋白质组影响的研究》由会员上传分享,免费在线阅读,更多相关内容在学术论文-天天文库。
1、湖南师范大学硕士学位论文递增运动负荷训练对大鼠心房肌蛋白质组影响的研究姓名:龚丽申请学位级别:硕士专业:运动人体科学指导教师:史绍蓉20070501AbstractThepurposeofthisstudyistodiSCUSStheinfluencewhichauricularmuscleproteonncsOnincreasingprogressivelyexercise‘load。trammg,sievetargetedproteinofvariabilityexpressedandimportantsignificanceinsport,investigationruleof
2、developmentandefflUenCeofsplortsfatiguemproteome.Applyingtheoryandtechniqueofproteome,tenmaleSDratsweredividedintotwogroupsrandomly:therestgroup(5)andtheexercisegroup(5).Afterincreasingprogressivelyexercise-load-training7weeks,exhaustingtraining6hours,theywerenarcotizationexecutedatthesalnetime
3、.Extractedwholeproteinofauricularmuscle,theproteinswereseparatedby2-DE,stainedbyCoomassiebrilliantbluedye,analyzedbyMALDI.TOF—TOF.MSinturn.354+40and382士24proteinspotswerefoundinthesportgroupandtestgroup,matclfingratewere68.67%士9.87%and72.67%士8.62%.coefficientcorrelationwere0。7l:illthe104differe
4、ntiallyexpressedspotsaftertraning,66inup-regulation2times,including18inup-regulation5times,noneproteinspotinincrease;38indown-regnlation2times,including23indown-regulation5times,7inobsolescented.Selectedproteinspotsofdifferentialexpressedmore5timesasobjectprotemum,therewremasschromatograptncana
5、lysis,5proteinspotswereassessmented:myosinlightpolypeptide4andaspartateaminotransferasewereup-regulation,Myosin-6Wasdown-regulationandabsence.Experimentalresultindicatedthatauricularmuscleproteinwereobviouslyvariabilityexpressed,anditWaSsufficientexperimentbaseinordertoprofoundlystudyoriginalob
6、jectiveproteinumofsportsfatigue.Theauricularmuscleproteinofvariabilityexpressedarerelatedtosignaltransduction,energymetabolism,substancemetabolismandmyocardialfunction.田lcdisappearedanddowm-regulatedproteinsinexercisegroupareralatedtodecreaseofmusclecontractionandmotorabilityandproceedofsportsf
7、atigue;theup-regulationareralatedtoinjuryofhistiooyticinlong-termmereasmgprogressivelyexercise。load-trmmng。Mostimportant,myosinlightpolypeptide4andaspartateaminotransferasethatsuccessfullysievethroughmethodofproteomewillimpactorga
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