氟比洛芬酯对脊髓P物质及NK1受体表达的影响

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1、上海交通大学硕士学位论文氟比洛芬酯对脊髓P物质及NK1受体表达的影响姓名：毛豪丽申请学位级别：硕士专业：麻醉学指导教师：姜虹20080501效果，可使痛阈升高,并可降低脊髓中SP及NK1-R的蛋白和mRNA表达。关键词氟比洛芬酯，慢性缩窄性损伤，SP，NK1受体THEEFFECTOFSUBSTANCEPANDNEUROKININ-1RECEPTORSINTHESPINALDORSALHORNNEURONSTHERAPEUTICSWITHFLURBIPROFENAXETILABSTRACTObject:Toexploreth

2、efeasibilityofSubstanceP(SP)andNeurokinin-1receptors(NK1-R)inspinaldorsalhornneuronsbyusingFlurbiprofenAxetil(FA)totherapytheischiadicnervechronicconstrictioninjury(CCI).Method:themaleSDratswereramdomlydividedto4groupsand6ratsineachgroup.IngroupA,theratswithischia

3、dicnerveCCIweretreatedbyFA;theratswithischiadicnerveCCIwereingroupB;IngroupC,theratswithshameoperationweretreatedbyFA;theratswithshameoperationwereingroupD.TherightischiadicnerveofratsinCCIgroupweredeligated,whiletheischiadicnerveofratsintheshamoperationgroupwereo

4、nlyexplored.Atthe15thdaypost-operation,5mg/KgFAweregiventoAandCgroupsbutnothingtocontrolgroupandthepainthresholdweremeasured.Theratsweresacrificedafter6hoursofadministrationtoacquirethethespinalcord.Thenthesampleswereundertakenimmunohistochemistry,immunofluorescen

5、ceandRT-PCRdetectionofSPandNK1-R.Moreover,histomorphometrywereusedtoquantifythedata.AlltheresultswerestatisticallyanalyzedbySPSS11.0software.Apvaluelessthan0.05wasconsideredstatisticallysignificant.Result：TheratsgothigherpainthresholdingroupAthaningroupB.Therewass

6、ignificantdifferencebetweenthesetwogroups.NosignificantdifferencewasfoundbetweengroupCandgroupDintermofthepainthreshold.Immunohistochemistry,immunofluorescenceandRT-PCRresultsshowedweakpositiveSP、NK1-RproteinandmRNAexpressioningroupA,whiletherewasstrongpositiveexp

7、ressioningroupB.Histomorphometryresultsshowedsignificantdifferencebetweenthesetwogroups.However,weakpositiveSP、NK1-RproteinandmRNAexpressionoccurredinbothgroupCandD,alsonosignificantdifferencewasfound.ConclusionObviousanalgesiaeffectivenessoccurredwithincreasedpai

8、nthresholdwhenusingFlurbiprofenAxetiltotreatratischiadicnervechronicconstrictioninjurymodel.ThismethodcouldalsodepresstheproteinandmRNAexpressionofSP、NK