广东与湖南养殖鳜群体遗传多样性研究

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1、华南师范大学硕士学位论文答辩合格证明学位申请人杏c涨向本学位论文答辩委员会提交题走j兰基复查蝤醴睦鲎釜鲢辞蟀溘良錾鞋塑军鍪幽硕士论文，经答辩委员会审议，本论文答辩合格，特此证明。学位论文答辩委员会委员(签名)主席：委员：犟卜雄盏一论文指导老师(签名)：五猛!垒加矿年6月扩日广东与湖南养殖鳜群体遗传多样性研究摘要Abstract延伸6rain。从40个随机引物中筛选出22个有效引物，共扩增产生289条RAPD标记片段，广东养殖群体共产生155条扩增片段，湖南养殖群体共产生134条扩增片段，有122条带为两群体共有。广东和湖南养殖群体内平均遗传距离分别为O．0633和0．0629，两群体间遗

2、传距离为0．1299。广东和湖南养殖群体Shannon多样性值分别为0．0690和O．0723，多态座位比例分别为25．16％和10．15％。并与脊椎动物平均多态座位比例比较，发现其遗传多样性并不很丰富。因此需要在生产过程中要采取行之有效的管理措施以避免或减少遗传多样性水平的降低，确保鳜养殖业的可持续发展。关键词：鳜(SinipercachuatsiBasilewsky)，核型，同工酶，随机扩增多态性DNA(RAPD)，遗传多样性￡变兰塑塑妻堕塑鲎堡望笪墨堂竺竺塞塑墨垒!!!!!竺ABSTRACTInChina，themandarinfish，SinipercachuatsiBasile

3、wsky(Perciformes：Serranidae)hasarelativelyhighmarketvalue，andiswidelyculturedthroughoutthecountry，withimportancealsoinstockingfisheriesinlakesandreservoirs，especiallyinGuangdongandHunanprovinces．StudyonthegeneticdiversityoffarmedmandarinfishstocksinGuangdong&Hunanusingkaryotype，allozymeandRAPDana

4、lyses，hasimportantcontent，atthesametime，protectsfishgeneticdiversityandgermplasmresources，promotessustainableutilityofbioresources．KaryotypesoffarmedmandarinfishstocksinGuangdong＆HunanweleobtainedfromkidneycellsafterinvivotreatmentwithPHAandcolchicineinjection—airdryingmethod．Theresultsshowedthat

5、thereweresamekaryotypeformulaas2n=48=24sm，st+24t，NF=70，consistingof22submetacentric，2subtelocentricand24telocentricpairs．Inbothmaleandfemaleofthestocksstudies，thespecialbig，small-chromosomesorheteromorphicsex—chromosomeswerenotfound．Thepossibleevolutionaryrelationsinchromosomearediscussed．Compara

6、tivestudiesofgeneticdiversityoffarmedmandarinfishflocksinGuangdong＆Hunanwereinvestigatedbypolyacrylamidgelelectrophoresis(PAGE)．Among22lociforthe7enzymesexamined．Threeloci，whichwereADH，MDH一2andMDH·3werefoundploymorphicinGuangdongstock，themeanproportionsofploymorphiclociwas13．64％．Twoloci，whichwere

7、MDH-2andMDH-3werefoundploymorphicinHunanstock，themeanproportionsofploymorphiclociwas9．09％．TheresultsshowedthatthegeneticsimilarityandgeneticdistanCebetweentwostocksare0．9994and0．0006respectively．The3j二墨!i!塑鲞堕塑登笪望堡兰登丝!塞