柴胡疏肝散对夹尾应激大鼠脑和胃组织GASRmRNA和CCKARmRNA表达的影响

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1、柴胡疏肝散对夹尾应激大鼠脑和胃组织GASRmRNA和CCK-ARmRNA表达的影响作者：张钰琴，凌江红，梁纲，王道刚，农树雄，黄华，农伟东，李艳玲【摘要】　　目的探讨柴胡疏肝散对夹尾应激大鼠脑和胃GASRmRNA和CCK-ARmRNA表达的影响。方法将30只大鼠随机分为3组，正常对照组、模型组和柴胡疏肝散组，每组10只，采用慢性夹尾刺激方法制作慢性应激模型，分别给予生理盐水、柴胡疏肝散水煎剂灌胃，共4周。观察其一般情况及胃组织学变化，并用原位杂交方法检测大鼠脑和胃组织胃泌素受体（GASR）mRNA和胆囊收缩素受体A（CCK-AR）mRNA

2、的表达。结果①模型组出现了抑郁和消化不良行为表现,而柴胡疏肝散组则较不显著；②模型组胃黏膜均出现不同程度的糜烂和炎症反应，而柴胡疏肝散组则较轻；③模型组大鼠胃和脑组织上的GASRmRNA表达量较正常对照组有显著升高（均P<0.05）,柴胡疏肝散组则较模型组有显著降低（均P<0.05）。模型组大鼠胃组织CCK-ARmRNA表达较正常对照组显著升高（P<0.05），柴胡疏肝散组较模型组有显著降低（P<0.05）。结论柴胡疏肝散能改善慢性应激抑郁和消化不良行为，其机制可能与其能减低脑和胃组织GASRmRNA表达和减低胃组

3、织CCK-ARmRNA表达有关。【关键词】柴胡疏肝散；GASRmRNA；CCK-ARmRNA；夹尾应12　　Abstract：ObjectiveTostudytheeffectsofChaihushugansanonthegastricreceptormRNA(GASRmRNA)andCCK-AreceptormRNA(CCK-ARmRNA)expressioninstressratsinducedbyconstantlysqueezingtheirtails.Methods30ratswereselectedandrandomlydiv

4、idedinto3groups:controlgroup,modelgroupandChaihushugansangroup(eachn=10).Theanimalmodelofstresswasestablishedbyconstantlysqueezingtheirtailsfor4wkandmeanwhilesaline,Chaihushugansandecoctionwereadministeredrespectivelytothreegroupsbygavage.Theanimal'sgeneralsituationandpat

5、hologicalchangewereobservedeveryweek,andGASRmRNAandCCK-ARmRNAexpressionofratsinbrainandgastrictissuesweremeasuredfourweekslater.Results①Themodelgroupappeareddepressionanddyspepsia,themanifestationinChaihushugansandecoctionmaynotbesoobviouscomparedwithmodelgroup.②Modelgrou

6、pappeareddifferentmoderatestomachmucosaerosionandinflammationbuttheywerenotsoobviousinChaihushugansangroup.③TheGASRmRNAandCCK-ARmRNAexpressionofthemodelgroupratsinbrainandgastrictissueswereincreasedsignificantlythanthoseofcontrolgroup（P<0.05）,whileChaihushugansangroupw

7、assignificantlylowerthanmodel12group（P<0.05）.ModelgroupwassignificantlyhigherthancontrolgroupinCCK-ARmRNAofgastrictissue（P<0.05）.ConclusionChaihushugansancanimprovedepressionanddyspepsia，itsmechanismisrelatedtodecreasingexpressionofGASRmRNAinbrainandgastrictissuesan

8、dCCK-ARmRNAingastrictissneofrats.　　　　Keywords：Chaihushugansan;GASRmRNA;CCK-ARmRNA;Stress12　　应激是一