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1、上海交通大学硕士学位论文复方中药益气苓对高血压大鼠抗氧化作用及机制研究姓名：章樱申请学位级别：硕士专业：临床医学内科学（心血管病学）指导教师：强卫国20080501上海交通大学医学院硕士学位论文Hsp105,Hsp90及Pam等抗氧化相关基因的调控干预SHR的预后，这些基因是否可能成为改善高血压预后的介入靶点，尚需进一步研究。关键词自发性高血压大鼠,超氧化物歧化酶,丙二醛,8-异前列腺素F2a,基因芯片上海交通大学医学院硕士学位论文THEANTI-OXIDATIONEFFECTSOFCOMPOUNDTRADITI--ONALCHINESEMEDICINEY
2、IQILINGINHYPERTENSIVERATSABSTRACTObjectiveToinvestigatetheanti-oxidationmechanismofcompoundtraditionalChinesemedicineYiqiling,whichiscomposedofPoriacocos(Schw)WolfandPollenTea,onthespontaneouslyhypertensiverats（SHR）andtoexplorenewtargetforimprovingtheprognosisofhypertension.Method
3、10maleSHRwererandomlydividedintotwogroups:Treatedgroup(Yiqilinggroup,5rats)andcontrolgroup(5rats).Theplasmacontentofmaleicdiadehyde(MDA)andsuperoxidedismutase(SOD),andtheurinecontentof8-iso-prostaglandinF2a(8-iso-PGF2a)intwogroupsweremeasured.Examinationofthegeneexpressionprofilew
4、asanalyzedbyusingcDNAmicroarrayincluding12000ratgenes.3oftheanti-oxidationgenes(Hsp90,Hsp105andPAM)wereselectedtoundergoreal-timequantitativePCR.ResultsThe8-iso-PGF2aurinelevelandtheMDAplasmalevelwerelowerinYiqilinggroupthanincontrol,whereastheSODplasmalevelwashigherinYiqilinggrou
5、pthanincontrol,andallhavestatisticsignificance.Inthe上海交通大学医学院硕士学位论文Yiqilinggrouptheexpressionof354geneswereup-regulatedand121genesweredown-regulatedincomparisonwiththecontrolgroup.Thereal-timequantitativePCRresultsoftheHsp90,Hsp105andPamgeneswereconsistentwiththosebythemicroarraye
6、xamination.ConclusionThesedataindicatedthatYiqilinghavetheeffectofanti-oxidationbyincreasingSODlevelanddecreasingMDAlevel.YiqilingmayinterfereintheprognosisofSHRbyregulatingtheexpressionofHsp105,Hsp90andPamgenesandsoon.Moreresearchisneededtoestablishwhetherthesegenesarethenewtarge
7、tsforimprovingtheprognosisofhypertension.KEYWORDSspontaneouslyhypertensiverats,SOD,MDA,8-iso-PGF2a,cDNAmicroarray上海交通大学医学院硕士学位论文图片目录图1对照组与实验组高血压大鼠血浆总SOD测定结果··············································7图2对照组与实验组高血压大鼠血浆MDA测定结果·············································10图38-异前列腺
8、素F2a的标准曲线························
