大米草生产生物柴油和燃料乙醇的初步研究

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1、西南交通大学硕士学位论文大米草生产生物柴油和燃料乙醇的初步研究姓名：陈慧清申请学位级别：硕士专业：生物化学与分子生物学指导教师：张志斌;张卫20061101西南交通大学硕士研究生学位论文第ii页因此，利用大米草生产生物燃料对缓解因化石燃料短缺所带来豹能源危机具有重要意义，并能起到保护生态环境的作用。关键词：大米草；水解；浓硫酸；发酵；生物油脂；乙醇；生物燃料西南交通大学硕士研究生学位论文第i页AbstractTostudythemainpurposeabouttheutilizationofrenewablematerials，theconcentratedsulfuric

2、acidhydrolysisofCOlmlloncordgrasswasstudied．ThekeyfactorsaffectingthehydrolysisofcoimnoncordgrassbyconcentratedsulfuricacidwereoptimizedusingOrthogonalExperimentalDesign．Theproductionofbiolipid，ethanolandbiofuelwasstudiedbyfermentingthecommoncordgrasshydrolysate．Andthiskindofpapershavenotb

3、eenrepotted．Atfirst，theconcentratedsulfuricacidhydrolysiswasstudied．TheKeyfactors，theconcentrationofsulfuricacid“O％-80％)，thehydrolyzingtemperature(25℃一70℃)，thehydrolyzingtime(0．5-4h)，thegranularityofcommoncordgrass(notacross10meshes、lO一20meshes、2p40meshes、40-60meshes、across60meshes)andthes

4、olidcontentsolidcontent(5．o％，7．5％，1096、12．5％)，wereinvestigated．TooptimizehydrolysisconditionsIdesignedfivefactorsandfourlevelsorthogonalexperimentL¨(4

5、)andfourfactorsandthreelevelsorthogonalexperimentb(3‘)．Fromtheexperimentalresults，concentrationofsulfuricacid，hydrolyzingtemperatureandhydr

6、olyzingtimewerethemainf8trotsamongthem．Theoptimizedhydrolysisconditionsarethattheconcentrationofsulfuricacidis50％，thehydrolyzingtemperatureis50℃，thehydrolyzingtimeis60rain，thegranularityofco咖oncordgrassislO一20meshesandthesolidcontentisl：10

7、n．西南交通大学硕士研究生学位论文第ii页Usingtheyeaststrain弛odospondi锄．toruloidesAS2．1389fermentthecommoncordgrasshydrolysate．TheglucoseWaSalmostusedupandtheuserateoftheotherreducingsugarwas42％whenfermentationtimewas96hours．Andthefattycoefficientwas17．7％，theyieldofbiolipidwas6．49／lOOg(biolipid／commoncordgras

8、s)．Themainpartsofmethyl—etherificationoffattyacidsarebiodiesel．Therecombinantyeaststrain6508—127wastheyeaststrain6508recombinedandcouldfermentxylose．Usingtherecombinantyeaststrain6508—127fermentthecommoncordgrasshydrolysate．FromtheanalyticalHPLCchromatogramofc